High glucose: activating autophagy and affecting the biological behavior of human lens epithelial cells
High glucose: activating autophagy and affecting the biological behavior of human lens epithelial cells
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高糖：激活自噬并影响人晶状体上皮细胞的生物学行为
DOI：
10.18240/ijo.2019.07.02
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发表时间：
2019-07-18
影响因子：
1.4
通讯作者：
Lu, Pei-Rong
中科院分区：
文献类型：
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作者：
Li, Dan;Liu, Gao-Qin;Lu, Pei-Rong
目的：阐明高糖条件下自噬对人晶状体上皮细胞（HLECs）的影响。方法：用不同浓度的葡萄糖和3-甲基腺嘌呤（3-MA）培养HLECs； Western blotting和免疫荧光组织化学检测自噬相关蛋白LC3B的表达。采用划痕实验定量HLECs的迁移能力，实时聚合酶链式反应测定转化生长因子-β（TGF-β）的表达。结果：与5 mmol/L正常葡萄糖处理相比，40 mmol/L葡萄糖处理可显着增加HLECs中自噬体的生成，而0.375 mmol/L 3-MA处理可抑制HLECs自噬体的生成。 0.375 mmol/L 3-MA处理可显着抑制高糖诱导的HLECs迁移和HLECs中TGF-β的表达。结论：高糖暴露后自噬促进HLECs细胞迁移，并增加TGF-β的表达，这可能与糖尿病性白内障的发生有关。
AIM: To clarify the effect of autophagy on human lens epithelial cells (HLECs) under high glucose conditions.METHODS: HLECs were cultured with different concentrations of glucose and 3-methyladenine (3-MA); the expression of autophagy-related protein LC3B was detected by Western blotting and immunofluorescence histochemistry. The migration of HLECs was quantified by scratch wound assay and the expression of transforming growth factor-beta (TGF-beta) was measured by real-time polymerase chain reaction.RESULTS: Compared with 5 mmol/L normal glucose treatment, 40 mmol/L glucose treatment can significantly increase the generation of autophagosome in HLECs, which could be inhibited by 0.375 mmol/L 3-MA treatment. The migration of HLECs and the expression of TGF-beta in HLECs induced by high glucose were significantly suppressed by 0.375 mmol/L 3-MA treatment.CONCLUSION: Autophagy promotes HLECs cell migration and increases the expression of TGF-beta after exposed to high glucose, which may relate to the development of diabetic cataract.