Towards detection and identification of circulating tumour cells using Raman spectroscopy
Towards detection and identification of circulating tumour cells using Raman spectroscopy
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DOI：
10.1039/c0an00608d
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发表时间：
2010-01-01
期刊：
影响因子：
4.2
通讯作者：
Popp, J.
中科院分区：
文献类型：
--
作者：
Neugebauer, U.;Bocklitz, T.;Popp, J.
体液是很容易获得的，并含有有价值的医学诊断指标。细胞因子是在癌症患者外周血中循环的肿瘤细胞。由于这些细胞极其罕见，它们对临床诊断构成了挑战。在这篇文章中，我们提出了基于拉曼光谱的鉴定悬浮液中的不同单细胞，这些细胞在癌症患者的外周血中发现，包括健康细胞如白细胞和红细胞，以及肿瘤细胞如白血病细胞和源自实体瘤的细胞。从健康供体的外周血中分离白细胞和红细胞，同时从细胞培养物中获得髓性白血病细胞（OCI-AML 3）和乳腺癌衍生细胞（MCF-7，BT-20）。发射785 nm光的激光器用于光学捕获激光焦点中的单细胞并激发拉曼光谱。应用支持向量机开发了一个监督分类模型，该模型具有来自三个不同供体和三个独立培养批次的1210个细胞的光谱。区分肿瘤细胞和健康细胞的灵敏度>99.7%，特异性> 99.5%。此外，正确的细胞类型预测的准确率约为92%。
Body fluids are easily accessible and contain valuable indices for medical diagnosis. Fascinating tools are tumour cells circulating in the peripheral blood of cancer patients. As these cells are extremely rare, they constitute a challenge for clinical diagnostics. In this contribution we present the Raman spectroscopic-based identification of different single cells in suspension that are found in peripheral blood of cancer patients including healthy cells like leukocytes and erythrocytes, and tumour cells like leukaemic cells and cells originating from solid tumours. Leukocytes and erythrocytes were isolated from the peripheral blood of healthy donors while myeloid leukaemia cells (OCI-AML3) and breast carcinoma derived cells (MCF-7, BT-20) were obtained from cell cultures. A laser emitting 785 nm light was used for optical trapping the single cells in the laser focus and to excite the Raman spectrum. Support vector machines were applied to develop a supervised classification model with spectra of 1210 cells originating from three different donors and three independent cultivation batches. Distinguishing tumour cells from healthy cells was achieved with a sensitivity of >99.7% and a specificity of >99.5%. In addition, the correct cell types were predicted with an accuracy of approximately 92%.