Changes in fungal community and diversity in strawberry rhizosphere soil after 12 years in the greenhouse
Changes in fungal community and diversity in strawberry rhizosphere soil after 12 years in the greenhouse
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DOI：
10.1016/s2095-3119(18)62003-9
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发表时间：
2019-03-01
影响因子：
4.8
通讯作者：
Liu Qi-zhi
中科院分区：
文献类型：
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作者：
Li Wei-hua;Liu Qi-zhi
土壤真菌在土壤生态环境中起着非常重要的作用。在农业生产中，长期的单作和连作导致真菌群落多样性的变化。然而，长期单作和连作对草莓植株健康和真菌群落多样性的影响尚未阐明。本研究采用高通量测序技术（HTS），比较了不同连作年限（0、2、4、6、8、10和12年）草莓根际土壤真菌群落结构和多样性。结果表明，土壤真菌多样性随连作年限的增加而增加。具体而言，土壤传播的疾病病原体镰刀菌和Guehomesturia草莓连作后显着增加，杀线虫（节肢动物）真菌的丰度从连作第四年下降。相关性分析结果表明，这3个属可能是影响连作土壤性质变化的关键真菌。此外，土壤理化性质分析表明，连作4年后土壤养分含量开始下降。斯皮尔曼的相关分析表明，土壤pH值，速效钾（AK）和铵态氮（NH 4 +-N）是最重要的土壤因子，导致对比的有益和病原协会在草莓连作制度。
Soil fungi play a very important role in the soil ecological environment. In agricultural production, long-term monoculture and continuous cropping lead to changes in fungal community diversity. However, the effects of long-term monoculture and continuous cropping on strawberry plant health and fungal community diversity have not been elucidated. In this study, using high-throughput sequencing (HTS), we compared the fungal community and diversity of strawberry rhizosphere soil after various durations of continuous cropping (0, 2, 4, 6, 8, 10 and 12 years). The results showed that soil fungal diversity increased with consecutive cropping years. Specifically, the soil-borne disease pathogens Fusarium and Guehomyces were significantly increased after strawberry continuous cropping, and the abundance of nematicidal (Arthrobotrys) fungi decreased from the fourth year of continuous cropping. The results of correlation analysis suggest that these three genera might be key fungi that contribute to the changes in soil properties that occur during continuous cropping. In addition, physicochemical property analysis showed that the soil nutrient content began to decline after the fourth year of continuous cropping. Spearman's correlation analysis showed that soil pH, available potassium (AK) and ammonium nitrogen (NH4+-N) were the most important edaphic factors leading to contrasting beneficial and pathogenic associations across consecutive strawberry cropping systems.