Aging, mating, and the evolution of mtDNA heteroplasmy in Drosophila melanogaster
Aging, mating, and the evolution of mtDNA heteroplasmy in Drosophila melanogaster
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DOI：
10.1073/pnas.95.5.2372
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发表时间：
1998-03-03
影响因子：
11.1
通讯作者：
Rand, DM
中科院分区：
文献类型：
--
作者：
Kann, LM;Rosenblum, EB;Rand, DM
异质性是指细胞内存在一种以上类型的线粒体DNA，在动物中普遍存在，并与人类的衰老和疾病有关，细胞和动物世代之间线粒体DNA变异频率的变化因此与线粒体DNA的进化和多种病理的进展有关。我们用个体异质果蝇的Southern杂交密度法研究了年龄、交配后产卵量的增加和温度对世代内和世代之间异质性进化的影响。18个独立的异质性母亲产生的F-1后代的早期和晚期mtDNA变异频率随着年龄的增长而持续增加，无论是温度(保持在25摄氏度和18摄氏度)还是在交配前保持处女10天对传播模式都没有显著影响，然而，在它们生命的尽头，在25摄氏度产卵的果蝇比在18摄氏度产卵的同胞果蝇具有更高的长mtDNA频率，研究了交配或作为处女的兄弟姐妹在一代内的异质性演变，然后在两个不同年龄(第2天和第14天)对mtDNA单倍型频率进行评分，交配果蝇显示出长mtDNA变异频率随年龄的增加显著高于未交配果蝇，该系统为联合分析世代和年龄段分子多态的传递动力学提供了一个模型。
Heteroplasmy, the presence of more than one type of mtDNA within cells, is common in animals and has been associated with aging and disease in humans, Changes in the frequencies of mtDNA variants between cell and animal generations thus bears on the evolution of mtDNA and the progression of diverse pathologies. We have used densitometry of Southern blots of individual heteroplasmic Drosophila melanogaster to study the effects of age, increased egg production after mating, and temperature on evolution of heteroplasmy within and between generations, The frequency of the longer mtDNA variant consistently increased between early and late cohorts of F-1 offspring derived from 18 independent heteroplasmic mothers as they aged, Neither temperature (flies maintained at 25 degrees C and 18 degrees C) nor the holding of flies as virgins for 10 days before mating had significant effects on transmission patterns, However, at the ends of their lives, flies that had laid eggs at 25 degrees C had a greater frequency of the long mtDNA than did their siblings who had laid eggs at 18 degrees C, The evolution of heteroplasmy within a generation was studied in samples of siblings that either were mated or held as virgins, and then scored for mtDNA haplotype frequencies at two different ages (day 2 and day 14), Mated flies showed a significantly greater increase in the frequency of the long mtDNA variant with age than did the unmated flies, This system provides a model for the joint analysis of generational and chronological age in the transmission dynamics of a molecular polymorphism.