Effects of bovine lactoferrin on in vitro replication of feline herpesvirus
Effects of bovine lactoferrin on in vitro replication of feline herpesvirus
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DOI：
10.1046/j.1463-5224.2003.00301.x
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发表时间：
2003-09-01
影响因子：
1.6
通讯作者：
Clarke, HE
中科院分区：
文献类型：
--
作者：
Beaumont, SL;Maggs, DJ;Clarke, HE
目的 研究牛乳铁蛋白对猫疱疹病毒 (FHV-1) 体外复制的影响，并确定这些影响在病毒复制过程中的哪个点发生。样本群体培养的 Crandell-Reese 猫肾 (CRFK) 细胞和 FHV-1 株 727。 程序 按三种浓度中的一种或多种添加五种浓度的牛乳铁蛋白（0.5、1、2、5 和 10 mg/mL）传统空斑减少测定中的时间点：(a)在病毒吸附之前将未感染的CRFK细胞在含乳铁蛋白的培养基中孵育30分钟； (b) 在吸附之前和过程中将病毒悬浮在含乳铁蛋白的培养基中，或 (c) 在病毒吸附后将 CRFK 细胞与含乳铁蛋白的培养基一起孵育 48 小时。对噬菌斑进行计数，并将抗病毒效果表示为相对于不含乳铁蛋白的对照培养基的抑制百分比。 结果在病毒吸附之前或过程中将CRFK细胞暴露于乳铁蛋白可抑制FHV-1复制87-96%（平均值：91%）。病毒吸附后应用乳铁蛋白对 FHV-1 复制没有明显影响。当乳铁蛋白分多个步骤添加时，没有发现相加或协同效应。这些效果在所有测试的乳铁蛋白浓度下都是相似的。在任何测试浓度下均未观察到乳铁蛋白对CRFK细胞的细胞毒性作用。结论和临床相关性牛乳铁蛋白在病毒吸附之前和过程中对FHV-1的体外复制具有显着的抑制作用，但在病毒吸附之后则没有这种作用。这些发现强烈表明乳铁蛋白抑制 FHV-1 吸附到细胞表面和/或病毒渗透到细胞中。局部乳铁蛋白对猫急性或复发性疱疹发作的临床效果值得研究。
Objective To investigate the effects of bovine lactoferrin on in vitro replication of feline herpes virus (FHV-1) and to determine at what points during viral replication these effects occur.Sample population Cultured Crandell-Reese feline kidney (CRFK) cells and FHV- 1 strain 727.Procedure Five concentrations of bovine lactoferrin (0.5, 1, 2, 5, and 10 mg/mL) were added at one or more of three time points during conventional plaque reduction assays: (a) uninfected CRFK cells were incubated in lactoferrin-containing medium for 30 min prior to viral adsorption; (b) virus was suspended in lactoferrin-containing medium prior to and during adsorption, or (c) CRFK cells were incubated with lactoferrin-containing medium for 48 h following viral adsorption. Plaques were counted and antiviral effect expressed as percent inhibition relative to control medium that contained no lactoferrin.Results Exposure of CRFK cells to lactoferrin prior to or during viral adsorption inhibited FHV-1 replication by 87-96% (mean: 91%). Application of lactoferrin following viral adsorption had no appreciable effect on FHV-1 replication. No additive or synergistic effects were noted when lactoferrin was added at multiple steps. These effects were similar at all concentrations of lactoferrin tested. Cytotoxic effects of lactoferrin on CRFK cells were not observed at any concentration tested.Conclusions and clinical relevance Bovine lactoferrin has a notable inhibitory effect on the in vitro replication of FHV- 1 prior to and during, but not following viral adsorption. These findings strongly suggest that lactoferrin inhibits FHV- 1 adsorption to the cell surface and/or penetration of the vir-us into the cell. Clinical effects of topical lactoferrin in acute or recrudescent herpetic episodes in cats warrant investigation.