The engraftment of transplanted bone marrow-derived cells into the olfactory epithelium
The engraftment of transplanted bone marrow-derived cells into the olfactory epithelium
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DOI：
10.1016/j.brainres.2005.05.061
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发表时间：
2005-08-02
期刊：
影响因子：
2.9
通讯作者：
Nagai, N
中科院分区：
文献类型：
--
作者：
Tsujigiwa, H;Nishizaki, K;Nagai, N
为了研究骨髓细胞是否迁移并植入嗅上皮并分化为嗅觉神经元，将绿色荧光蛋白（GFP）小鼠的骨髓细胞移植到经致死照射的受体小鼠中。进行免疫组织化学染色以评估供体骨髓细胞植入嗅上皮的情况。骨髓重建后 2 周，最初在嗅觉上皮中发现了 GFP 的免疫染色。骨髓重建后 12 个月内，GFP 阳性细胞的百分比增加。 GFP 和嗅觉标记蛋白的双重染色显示 GFP 阳性细胞群具有嗅觉神经元的特征。这些结果表明骨髓细胞可以移植到嗅上皮中，然后分化为嗅神经元细胞。 (c) 2005 Elsevier B.V. 保留所有权利。
To investigate whether bone marrow cells migrate and are engrafted into the olfactory epithelium and differentiate into olfactory neurons, bone marrow cells of green fluorescence protein (GFP) mice were transplanted into lethally irradiated recipient mice. Immunohistochemical staining was performed to evaluate the engraftment of donor bone marrow cells into the olfactory epithelium. Immunostaining for GFP was found initially in the olfactory epithelium 2 weeks after bone marrow reconstruction. The percentage of GFP positive cells increased up to 12 months after bone marrow reconstruction. Double staining for GFP and olfactory marker protein showed that a population of the GFP-positive cells had characteristics of olfactory neurons. These results demonstrate that bone marrow cells can be engrafted in the olfactory epithelium and then differentiate into olfactory neuron cells. (c) 2005 Elsevier B.V. All rights reserved.