Identification of Tertiary Lymphoid Structure-Associated Follicular Helper T Cells in Human Tumors and Tissues
Identification of Tertiary Lymphoid Structure-Associated Follicular Helper T Cells in Human Tumors and Tissues
复制标题
DOI：
10.1007/978-1-4939-8709-2_12
复制
发表时间：
2018-01-01
期刊：
影响因子：
--
通讯作者：
Kaplon, Helene
中科院分区：
文献类型：
--
作者：
Couillault, Coline;Germain, Claire;Kaplon, Helene
滤泡辅助 T (Tfh) 细胞是体液免疫反应的主要组成部分，因为它们在 B 细胞同种型转换后的生发中心形成和抗体亲和力成熟中发挥着关键作用。这种CD4(+) T细胞亚型主要存在于二级淋巴器官的B细胞区以及三级淋巴结构(TLS)中，三级淋巴结构是由T细胞和B细胞组成的高度组织化的结构，偶尔在肿瘤微环境的侵袭边缘发现。我们在此描述如何对肿瘤组织切片进行免疫荧光染色并对肿瘤细胞悬浮液进行多色流式细胞术，以识别和可视化各种人类癌症的肿瘤微环境中的这些与TLS相关的Tfh细胞。这些测定利用了参与 Tfh 分化和功能的标记物和分子的组合。
Follicular helper T (Tfh) cells are major components of the humoral immune response due to their pivotal role in germinal center formation and antibody affinity maturation following B-cell isotype switching. This CD4(+) T-cell subtype is mainly found in the B-cell zone of secondary lymphoid organs as well as in tertiary lymphoid structures (TLS), which are highly organized structures composed of T and B cells, occasionally found at the invasive margin in the tumor microenvironment.We describe here how to perform immunofluorescence staining of tumor tissue sections and multicolor flow cytometry on tumor cell suspensions to identify and visualize these TLS-associated Tfh cells within the tumor microenvironment of various human cancers. These assays take advantage of combinations of markers and molecules involved in Tfh differentiation and function.