Behavioral profile of rats submitted to session 1-session 2 in the elevated plus-maze during diurnal/nocturnal phases and under different illumination conditions
Behavioral profile of rats submitted to session 1-session 2 in the elevated plus-maze during diurnal/nocturnal phases and under different illumination conditions
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DOI：
10.1016/s0166-4328(01)00396-5
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发表时间：
2002-05-14
影响因子：
2.7
通讯作者：
Carobrez, AP
中科院分区：
文献类型：
--
作者：
Bertoglio, LJ;Carobrez, AP
高架十字迷宫（高架十字迷宫）模型通常采用夜行物种（例如大鼠和小鼠），并且测试几乎完全在日间阶段（开灯）进行，导致一些实验室在反向光周期下用动物进行实验以克服这个问题。然而，这是值得怀疑的人工逆转的光周期短的时间内是否保证在正常受试者中发现的所有生理参数的修改。本研究评价了在不同光照条件下大鼠在正常昼夜或夜间阶段的第1- 2阶段（S1-S2）的光吸收曲线。在S2中，无论昼夜节律阶段和照明条件如何，所有组的既往暴露均减少了开臂探索;然而，与日间阶段相比，在夜间阶段测试的受试者中，这种行为减少。风险评估（RA）的行为降低在高照明下的两个昼夜节律阶段在SI和增加在第一分钟的S2，当与SI的最后一分钟相比。虽然开臂探索和RA行为在高照明下减少，但与两个昼夜节律阶段的低照明条件相比，一般自发活动仅在夜间阶段减少。结果进行了讨论，在行为特征的昼夜变化，并作为一个可能的来源，在临床前的焦虑模型的变异。(C)2002 Elsevier Science B. V.保留所有权利。
The elevated plus-maze (EPM) model usually employs nocturnal species (e.g. rats and mice) and the tests are almost exclusively performed during the diurnal phase (lights on), leading some laboratories to perform experiments with animals under a reversed light cycle to overcome this problem. However, it is questionable whether the artificial reversal of the light cycle for short periods guarantees modifications in all the physiological parameters found in normal subjects. The present study evaluated the session 1-session 2 (S1-S2) EPM profile in rats during their normal diurnal or nocturnal phase using different illumination conditions. Prior exposure to the EPM decreased open arm exploration for all groups in S2, regardless of the circadian phase and illumination condition; however, this behavior was decreased in subjects tested during the nocturnal phase, when compared to the diurnal phase. Risk assessment (RA) behavior was decreased under high illumination for both circadian phases in SI and increased in the first minute of S2, when compared to the last minute of SI. Although open arm exploration and RA behavior were decreased under high illumination, when compared to low illumination conditions in both circadian phases, general locomotor activity was only decreased during the nocturnal phase. The results are discussed in terms of circadian variations in the behavioral profile and as a possible source of variability in pre-clinical models of anxiety. (C) 2002 Elsevier Science B.V. All rights reserved.