INTERDEPENDENCE OF COENZYME-INDUCED CONFORMATIONAL WORK AND BINDING POTENTIAL IN YEAST ALCOHOL AND PORCINE HEART LACTATE-DEHYDROGENASES - A HYDROGEN-DEUTERIUM EXCHANGE STUDY
INTERDEPENDENCE OF COENZYME-INDUCED CONFORMATIONAL WORK AND BINDING POTENTIAL IN YEAST ALCOHOL AND PORCINE HEART LACTATE-DEHYDROGENASES - A HYDROGEN-DEUTERIUM EXCHANGE STUDY
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DOI：
10.1021/bi00389a026
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发表时间：
1987-07-28
期刊：
影响因子：
2.9
通讯作者：
KAGI, JHR
中科院分区：
文献类型：
--
作者：
DEWECK, Z;PANDE, J;KAGI, JHR
材料与方法雅得冻干粉购自勃林格公司。PHLDH是从Sigma中得到的结晶悬浮液，在1.9M硫酸铵中，pH为6。从Boehringer得到NAD+和NADH。ADP-核糖、ADP和AMP来自Sigma，吡唑来自Aldrich。用于紫外光谱的丙酮和异丙醇购自福陆卡。购买草酸作为钾盐1缩写：YADH，酵母酒精脱氢酶；LDH，来源不明的乳酸脱氢酶；DFLDH，角鱼乳酸脱氢酶；PHLDH，猪心乳酸脱氢酶；CHLDH，鸡心乳酸脱氢酶；GAPDH，甘油醛-3-磷酸脱氢酶；LADH，肝醇脱氢酶；GDH，谷氨酸-塔酸脱氢酶，MDH，苹果酸脱氢酶，HD交换，氢-氘交换。
Materials and Methods YADH was purchased as a lyophilized powder from Boehringer. PHLDH was obtained from Sigma as a crys-talline suspension in 1.9 M ammonium sulfate, pH 6. NAD+ and NADH were obtained from Boehringer. ADP-ribose, ADP, and AMP were from Sigma, and pyrazole was from Aldrich. Acetone and 2-propanolfor UV spectroscopy were purchased from Fluka. Oxalate was purchased as potassium1 Abbreviations: YADH, yeast alcohol dehydrogenase; LDH, lactate dehydrogenase of unspecified origin; DFLDH, dogfish lactate dehydrogenase; PHLDH, porcine heart lactate dehydrogenase; CHLDH, chicken heart lactate dehydrogenase; GAPDH, glyceraldehyde-3-phos-phate dehydrogenase; LADH, liver alcohol dehydrogenase; GDH, glu-tamate dehydrogenase, MDH, malate dehydrogenase, HD exchange, hydrogen-deuterium exchange.