DNA Hydrogel with Tunable pH-Responsive Properties Produced by Rolling Circle Amplification
DNA Hydrogel with Tunable pH-Responsive Properties Produced by Rolling Circle Amplification
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通过滚环扩增产生具有可调 pH 响应特性的 DNA 水凝胶
DOI：
10.1002/chem.201704390
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发表时间：
2017-12-22
影响因子：
4.3
通讯作者：
Tian, Leilei
中科院分区：
文献类型：
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作者：
Xu, Wanlin;Huang, Yishun;Tian, Leilei
近年来，由寡核苷酸自组装或寡核苷酸-聚合物杂化物交联形成的智能DNA水凝胶引起了人们的极大关注。然而，DNA水凝胶制备的困难限制了其实际应用。本文报道了一种通过滚环扩增（RCA）制备pH响应性水凝胶的新方法。在该方法中，在DNA合成过程中将pH敏感的交联位点引入聚合DNA链中。由于DNA序列可以通过其模板精确定义，因此可以通过模板设计以非常容易的方式微调这种水凝胶的性质。我们已经研究了水凝胶形成和pH响应性的过程，以提供基于RCA反应的功能性水凝胶设计的依据。
Recently, smart DNA hydrogels, which are generally formed by the self-assembly of oligonucleotides or through the cross-linking of oligonucleotide-polymer hybrids, have attracted tremendous attention. However, the difficulties of fabricating DNA hydrogels limit their practical applications. We report herein a novel method for producing pH-responsive hydrogels by rolling circle amplification (RCA). In this method, pH-sensitive cross-linking sites were intro-duced into the polymeric DNA chains during DNA synthesis. As the DNA sequence can be precisely defined by its template, the properties of such hydrogels can be finely tuned in a very facile way through template design. We have investigated the process of hydrogel formation and pH-responsiveness to provide rationales for functional hydrogel design based on the RCA reaction.