Structural insights into electron transfer in caa3-type cytochrome oxidase.
Structural insights into electron transfer in caa3-type cytochrome oxidase.
复制标题
caa3 型细胞色素氧化酶中电子转移的结构见解。
DOI：
10.1038/nature11182
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发表时间：
2012-07-26
期刊：
影响因子：
64.8
通讯作者：
Caffrey, Martin
中科院分区：
文献类型：
--
作者：
Lyons, Joseph A.;Aragao, David;Slattery, Orla;Pisliakov, Andrei V.;Soulimane, Tewfik;Caffrey, Martin
细胞色素c氧化酶是血红素铜氧化酶超家族（HCO）的成员。HCO作为线粒体和需氧原核生物呼吸链中的末端酶，将分子氧还原与跨膜质子泵联用。酶功能的组成部分是通过两种蛋白质的瞬时结合将电子从细胞色素c转移到氧化酶。电子的进入和退出被认为发生在细胞色素c的同一位点。在这里，我们报告的CaA 3型细胞色素氧化酶从嗜热栖热菌，它有一个共价连接的细胞色素C结构域的晶体结构。使用合成的短链单酰基甘油作为宿主脂质，晶体在双连续中间相中生长。从电子密度图，在2.36 μ m的分辨率，一个新的整体膜亚基和一个天然的糖甘油磷脂嵌入在复杂的确定。与以前观察到的可溶性细胞色素c的电子转移机制相反，该结构揭示了融合的铜氧还蛋白/细胞色素c结构域的电子转移复合物的结构，该结构域暗示了细胞色素c上用于电子进入和退出的不同位点。支持替代经典的质子门特性，这HCO类。
Cytochrome c oxidase is a member of the heme copper oxidase superfamily (HCO). HCOs function as the terminal enzymes in the respiratory chain of mitochondria and aerobic prokaryotes, coupling molecular oxygen reduction to transmembrane proton pumping. Integral to the enzyme’s function is the transfer of electrons from cytochrome c to the oxidase via a transient association of the two proteins. Electron entry and exit are proposed to occur from the same site on cytochrome c. Here we report the crystal structure of the caa3-type cytochrome oxidase from Thermus thermophilus, which has a covalently tethered cytochrome c domain. Crystals were grown in a bicontinuous mesophase using a synthetic short-chain monoacylglycerol as the hosting lipid. From the electron density map, at 2.36 Å resolution, a novel integral membrane subunit and a native glycoglycerophospholipid embedded in the complex were identified. Contrary to previous electron transfer mechanisms observed for soluble cytochrome c, the structure reveals the architecture of the electron transfer complex for the fused cupredoxin/cytochrome c domain which implicates different sites on cytochrome c for electron entry and exit. Support for an alternative to the classical proton gate characteristic of this HCO class is presented.
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DOI：
10.1107/s0907444905036693
发表时间：
2006-01-01
影响因子：
2.2
作者：
Evans, P
通讯作者：
Evans, P
影响因子：
3.3
作者：
Klauda, Jeffery B.;Venable, Richard M.;Freites, J. Alfredo;O'Connor, Joseph W.;Tobias, Douglas J.;Mondragon-Ramirez, Carlos;Vorobyov, Igor;MacKerell, Alexander D., Jr.;Pastor, Richard W.
通讯作者：
Pastor, Richard W.
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
2.5
作者：
Fischetti, Robert F.;Xu, Shenglan;Smith, Janet L.
通讯作者：
Smith, Janet L.
影响因子：
3.8
作者：
Hoefer, Nicole;Aragao, David;Caffrey, Martin
通讯作者：
Caffrey, Martin