Genomic and structural features of the yellow fever virus from the 2016-2017 Brazilian outbreak
Genomic and structural features of the yellow fever virus from the 2016-2017 Brazilian outbreak
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DOI：
10.1099/jgv.0.001033
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发表时间：
2018-04-01
影响因子：
3.8
通讯作者：
Bonaldo, Myrna Cristina
中科院分区：
文献类型：
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作者：
Gomez, Mariela Martinez;Santos de Abreu, Filipe Vieira;Bonaldo, Myrna Cristina
自2016年底以来，巴西东南部一直遭受严重的森林黄热病病毒（YFV）爆发的快速扩张，该病毒已到达巴西和南美洲人口最多的地区之一，迄今为止，70多年来一直是黄热病无区。在目前的研究中，我们描述了来自2017年巴西疫情的蚊子，人类和非人类灵长类动物的12个YFV样本的完整基因组。所有YFV序列都属于南美洲基因型I的现代谱系（亚谱系1E），在2016年12月检测之前已经传播了几个月。我们的数据证实，与过去70年来南美最严重的YF流行相关的病毒株显示出独特的氨基酸替换，这些替换主要位于非结构蛋白的高度保守位置。我们的数据还证实，YFV已经向南传播到里约热内卢州，沿着两条主要的森林传播路线，这些路线在包括近1200万未接种疫苗的居民的大都市区边界汇合。我们的原始结果可以帮助公共卫生当局指导面临如此严重的流行病学问题所需的监测，预防和控制措施。最后，这也将启发其他工作者进一步研究巴西2017年YFV毒株中检测到的氨基酸多态性的流行病学和生物学意义。
Southeastern Brazil has been suffering a rapid expansion of a severe sylvatic yellow fever virus (YFV) outbreak since late 2016, which has reached one of the most populated zones in Brazil and South America, heretofore a yellow fever-free zone for more than 70 years. In the current study, we describe the complete genome of 12 YFV samples from mosquitoes, humans and non-human primates from the Brazilian 2017 epidemic. All of the YFV sequences belong to the modern lineage (sub-lineage 1E) of South American genotype I, having been circulating for several months prior to the December 2016 detection. Our data confirm that viral strains associated with the most severe YF epidemic in South America in the last 70 years display unique amino acid substitutions that are mainly located in highly conserved positions in non-structural proteins. Our data also corroborate that YFV has spread southward into Rio de Janeiro state following two main sylvatic dispersion routes that converged at the border of the great metropolitan area comprising nearly 12 million unvaccinated inhabitants. Our original results can help public health authorities to guide the surveillance, prophylaxis and control measures required to face such a severe epidemiological problem. Finally, it will also inspire other workers to further investigate the epidemiological and biological significance of the amino acid polymorphisms detected in the Brazilian 2017 YFV strains.