Synergistic ex-situ biodegradation of crude oil by halotolerant bacterial consortium of indigenous strains isolated from on shore sites of Gujarat, India
Synergistic ex-situ biodegradation of crude oil by halotolerant bacterial consortium of indigenous strains isolated from on shore sites of Gujarat, India
复制标题
DOI：
10.1016/j.ibiod.2015.03.030
复制
发表时间：
2015-09
影响因子：
4.8
通讯作者：
Sunita Varjani;Dolly Pal Rana;A. Jain;S. Bateja;V. Upasani
中科院分区：
文献类型：
--
作者：
Sunita Varjani;Dolly Pal Rana;A. Jain;S. Bateja;V. Upasani
石油工业活动造成的碳氢化合物污染是一个世界性的重大问题，特别是在像印度这样的发展中国家。碳氢化合物利用细菌联合体(HUBc)由六(06)个细菌分离物组成。Ochrobactrumsp.(01)、嗜麦芽窄食单胞菌(02)和铜绿假单胞菌(03)。用VITEK®2 COMPACT和16S rDNA基因测序对分离株进行鉴定。在优化的生长条件下进行了HUBc降解原油(K#X，3%v/v)的放大研究，最长可达75d。重量法和气相色谱分析表明，C8-C35的降解率分别为83.70%和83.49%。原油中的石蜡(C20-C40)在勘探活动中会造成油井堵塞问题。HUBc利用了原油中的石蜡含量，因此可以用来解决这一问题。由耐盐HUBc进行的FEX情景生物强化研究的结果表明，它在碳氢化合物污染场地的现场生物修复以及海面溢油管理方面具有有效的有效性。
Hydrocarbon pollution due to activities of petroleum industry is a major problem worldwide especially in developing countries like India. Hydrocarbon utilizing bacterial consortium (HUBC) consisting of six (06) bacterial isolatesviz. Ochrobactrumsp. (01),Stenotrophomonas maltophilia(02) andP. aeruginosa(03) isolated from crude oil polluted sites of ONGC fields of Gujarat, India. The isolates were identified by using VITEK®2 Compact and 16S rDNA gene sequencing. Scale up study of crude oil degradation (K#X, 3% v/v) by HUBC using Bushnell-Hass medium was performed under optimized growth conditions up to 75 days. Gravimetric and gas chromatographic analysis showed 83.70% and 83.49% (C8–C35) degradation, respectively. Presence of paraffins (C20–C40) in crude oil causes well clogging problem during exploration activities. The HUBC utilized paraffin content of crude oil and hence can be used to solve this problem. The findings ofex-situbioaugmentation studies by halotolerant HUBC suggests its utility for effectivein situbioremediation of hydrocarbon contaminated sites as well as management of sea surface oil spills.