Epidermal growth factor inhibits growth of A431 human epidermoid carcinoma in serum-free cell culture.
Epidermal growth factor inhibits growth of A431 human epidermoid carcinoma in serum-free cell culture.
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DOI：
10.1083/jcb.93.1.1
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发表时间：
1982-04
影响因子：
7.8
通讯作者：
Barnes, D W
中科院分区：
文献类型：
--
作者：
Barnes, D W
由补充有牛胰岛素（10微克/毫升）、人转铁蛋白（10微克/毫升）、人冷不溶性球蛋白（5微克/毫升）和乙醇胺（0.5 mM）的丰富的基础营养混合物组成的培养基支持A431人表皮样细胞系在不存在血清的情况下生长，其世代时间等于含血清培养基中细胞的世代时间。在此培养基中加入表皮生长因子（EGF）对其他细胞类型的有丝分裂浓度导致A431细胞生长的显着抑制。在1 ng/ml时观察到EGF的抑制作用，在10 ng/ml时观察到接近最大作用。EGF的抑制作用可以通过在随后的培养基更换中省略EGF来逆转，并且可以通过向培养基中加入抗EGF抗体来防止。EGF对A431细胞生长的抑制作用在含血清培养基中也能被证实。
A medium consisting of a rich basal nutrient mixture supplemented with bovine insulin (10 micrograms/ml), human transferrin (10 micrograms/ml), human cold-insoluble globulin (5 micrograms/ml), and ethanolamine (0.5 mM) supported the growth of the A431 human epidermoid cell line in the absence of serum with a generation time equal to that of cells in serum-containing medium. Addition of epidermal growth factor (EGF) to this culture medium at concentration mitogenic for other cell types resulted in a marked inhibition of A431 cell growth. Inhibitory effects of EGF were observed at 1 ng/ml and near-maximal effects were observed at 10 ng/ml. The inhibitory effect of EGF could be reversed by the omission of EGF in subsequent medium changes and could be prevented by the addition of anti-EGF antibody to the culture medium. Inhibition of A431 cell growth by EGF also could be demonstrated in serum-containing medium.