Fabrication of a novel bio-sorbent based on magnetic β-cyclodextrin composites modified by polymeric deep eutectic solvent for the efficient separation of Ovalbumin
Fabrication of a novel bio-sorbent based on magnetic β-cyclodextrin composites modified by polymeric deep eutectic solvent for the efficient separation of Ovalbumin
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一种基于磁性β-环糊精复合材料的新型生物吸附剂的制备，经聚合物低共熔溶剂改性，用于有效分离卵清蛋白
DOI：
10.1016/j.seppur.2021.118422
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发表时间：
2021-06
影响因子：
8.6
通讯作者：
Yuzhi Wang
中科院分区：
文献类型：
--
作者：
Yinyin Qian;Kaijia Xu;Rui Ni;Yuzhi Wang
本工作将β-环糊精接枝的磁性Fe3O4纳米粒子固定在聚合物深共晶溶剂(M-β-Cd@PDES)中，制备了一种新型的磁响应型吸附剂。X-射线衍射仪、振动样品磁强计、热重分析、傅里叶变换红外光谱、透射电子显微镜等测试手段证实了该吸附剂的成功制备。同时，通过间歇吸附实验考察了M-β-CD@PDES对卵清蛋白的吸附性能。结果表明，聚合物深度共熔溶剂与M-β-CD微球的偶联可显著提高其对卵白蛋白的吸附能力。此外，还对溶液pH值、OVA溶液浓度、吸附时间和温度等吸附参数进行了探索和优化。结果表明，M-β-Cd@PDES的最大吸附容量可达151.62 mg/g，溶液pH与Zeta电位的耦合分析表明，静电相互作用在吸附过程中起着重要作用。对M-β-Cd@PDES的再生进行了探索，结果表明，该吸附剂在三次吸附-脱附循环后仍具有一定的耐久性。此外，在SDS-PAGE分析的基础上，该吸附剂可用于从真实蛋清中吸附卵清蛋白。以上结果表明，M-β-Cd@PDES复合材料在生物分子分离方面具有很大的潜力。
In this work, a novel magnetically responsive sorbent is fabricated by immobilizing the β-cyclodextrin-grafted magnetic Fe3O4nanoparticles with polymeric deep eutectic solvents (M-β-CD@PDES). The successful preparation of the sorbent is confirmed by X-ray diffraction (XRD), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), Fourier transform infrared spectrometry (FT-IR) and transmission electron microscopy (TEM). Simultaneously, the adsorption performance of the M-β-CD@PDES for Ovalbumin (OVA) is investigated by batch adsorption experiments. The results reveal that the coupling of polymeric deep eutectic solvents with the M-β-CD microspheres could greatly enhances the adsorption capacity for OVA. Besides, the adsorption parameters in terms of solution pH value, the concentration of OVA solution, adsorption time and temperature are explored and optimized. It turns out that the maximum adsorption capacity can reach up to 151.62 mg/g. Meanwhile, the analysis of solution pH couple with the Zeta potentials of M-β-CD@PDES demonstrates that the electrostatic interaction plays a significant role in the adsorption procedure. The regeneration of M-β-CD@PDES is also explored and the result indicates the sorbent has certain durability after three adsorption–desorption cycles. Furthermore, on the basis of the analysis of SDS-PAGE, the sorbent can be applied to the adsorption of OVA from real egg white. All the above highlight the developed M-β-CD@PDES composites is supposed to have great potentials in the separation of biomolecules.
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TiO2@天冬氨酸-β-环糊精@还原氧化石墨烯光催化降解双酚A
影响因子：
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作者：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1039/c9an02556a
发表时间：
2020-02
期刊：
The Analyst
影响因子：
--
作者：
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通讯作者：
Xiaoxia Li;K. Row