IDENTIFICATION OF THE SIMIAN ROTAVIRUS SA11 GENOME SEGMENT-3 PRODUCT
IDENTIFICATION OF THE SIMIAN ROTAVIRUS SA11 GENOME SEGMENT-3 PRODUCT
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DOI：
10.1016/0042-6822(88)90230-9
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发表时间：
1988-03-01
期刊：
影响因子：
3.7
通讯作者：
ESTES, MK
中科院分区：
文献类型：
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作者：
LIU, M;OFFIT, PA;ESTES, MK
先前关于轮状病毒基因编码分配的研究未能清楚地识别猿猴轮状病毒SA11基因组片段3蛋白产物。通过使用新的电泳条件重新审视了这个问题，提高了高分子量范围内蛋白质的分辨率。我们的结果表明 SA11 基因组片段 3 编码表观摩尔重量为 88,000 的蛋白质。该蛋白通常与基因组片段 4 的蛋白产物共迁移。通过比较来自感染细胞和纯化的双壳、单壳和核心颗粒的 [35S] 蛋氨酸标记的病毒多肽的电泳模式，将基因 3 蛋白定位在病毒核心颗粒中。为了确认基因 3 产物的身份，我们还研究了两种重配病毒，其中基因组片段 3 是由两种亲本病毒株（SA11 和 NCDV）中的每一种重配而来。这些病毒的基因 3 和基因 4 产物通过以下方式鉴定：(i) 它们被两种不同的聚丙烯酰胺凝胶系统分离，(ii) 它们在不同颗粒类型中的位置，(iii) 它们对胰蛋白酶消化的不同敏感性，以及 (iv) 它们独特的蛋白酶肽图。我们建议从现在开始将基因组片段 3 的产物命名为 VP3，将基因组片段 4 的产物命名为 VP4。
Previous studies on rotavirus gene-coding assignments failed to clearly identify the simian rotavirus SA11 genome segment 3 protein product. This question was reexamined by using new conditions of electrophoresis with improved resolution of proteins in the high-molecular-weight range. Our results showed that the SA11 genome segment 3 codes for a protein with an apparent mol wt of 88,000. This protein normally comigrates with the protein product of genome segment 4. The gene 3 protein was located in viral core particles by comparing the electrophoretic patterns of [35S]methionine-labeled viral polypeptides from infected cells and from purified double-shelled, single-shelled, and core particles. To confirm the identity of the gene 3 product, we also studied two reassortant viruses in which genome segment 3 was reassorted from each of two parental virus strains (SA11 and NCDV). The gene 3 and gene 4 products of these viruses were identified by (i) their separation by two different polyacrylamide gel systems, (ii) their location in distinct particle types, (iii) their differential sensitivity to trypsin digestion, and (iv) their distinctive protease peptide maps. We propose that the genome segment 3 products be called VP3 and that the gene 4 product be named VP4 from now on.