EVIDENCE FOR LOCALIZED CELL HEATING INDUCED BY INFRARED OPTICAL TWEEZERS
EVIDENCE FOR LOCALIZED CELL HEATING INDUCED BY INFRARED OPTICAL TWEEZERS
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DOI：
10.1016/s0006-3495(95)80396-6
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发表时间：
1995-05-01
影响因子：
3.4
通讯作者：
TROMBERG, BJ
中科院分区：
文献类型：
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作者：
LIU, Y;CHENG, DK;TROMBERG, BJ
用红外光镊对脂质体和中国仓鼠卵巢（CHO）细胞进行限制，结果表明，通过一种无创的、空间分辨的荧光检测技术，样品加热和温度升高了好几摄氏度。对于微米大小的球形脂质体囊泡，其双层膜由磷脂1,2-二酰基-十五烷酰-甘油-磷脂胆碱（15-OPC）组成，当使用功率密度约为10(7)W/cm(2)、聚焦光斑大小约为0.8 μ m的1.064 μ m光镊固定囊泡时，观察到温度升高约为1.45 +/- 0.15℃/100 mW。样品温度的升高与应用光功率在40至250 mW范围内呈线性关系。在相同的捕获条件下，CHO电池的平均温升接近1.15 +/- 0.25℃/100 mW。红外镊子约束引起的细胞加热程度可以用热传导模型来描述，该模型分别考虑了水细胞核心和膜区域对红外激光辐射的吸收。观察到的结果与评估红外捕获光束在微操作应用和细胞生理学研究中的非侵入性有关。
The confinement of liposomes and Chinese hamster ovary (CHO) cells by infrared (IR) optical tweezers is shown to result in sample heating and temperature increases by several degrees centigrade, as measured by a noninvasive, spatially resolved fluorescence detection technique. For micron-sized spherical liposome vesicles having bilayer membranes composed of the phospholipid 1,2-diacyl-pentadecanoyl-glycero-phosphocholine (15-OPC), a temperature rise of similar to 1.45 +/- 0.15 degrees C/100 mW is observed when the vesicles are held stationary with a 1.064 mu m optical tweezers having a power density of similar to 10(7) W/cm(2) and a focused spot size of similar to 0.8 mu m. The increase in sample temperature is found to scale linearly with applied optical power in the 40 to 250 mW range. Under the same trapping conditions, CHO cells exhibit an average temperature rise of nearly 1.15 +/- 0.25 degrees C/100 mW. The extent of cell heating induced by infrared tweezers confinement can be described by a heat conduction model that accounts for the absorption of infrared (IR) laser radiation in the aqueous cell core and membrane regions, respectively. The observed results are relevant to the assessment of the noninvasive nature of infrared trapping beams in micromanipulation applications and cell physiological studies.