Characterization of desmoglein 2 expression in ovarian serous tumors and its prognostic significance in high-grade serous carcinoma
Characterization of desmoglein 2 expression in ovarian serous tumors and its prognostic significance in high-grade serous carcinoma
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桥粒芯糖蛋白2在卵巢浆液性肿瘤中的表达特征及其在高级别浆液性癌中的预后意义
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发表时间：
2018
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通讯作者：
Yixin Liu
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作者：
Ling Chen;Xinxin Liu;Jin Zhang;Yanxia Liu;Ang Gao;Yanning Xu;Yang Lin;Qiuyue Du;Zhengmao Zhu;Yuanjing Hu;Yixin Liu
桥粒糖蛋白（Desmogleins，Dsgs）是桥粒钙粘蛋白的主要成员，在上皮细胞的细胞粘附和正常组织结构的维持中起重要作用。DSG 2是最普遍存在的桥粒钙粘蛋白，然而，在几种类型的癌症中检测到DSG 2的异常表达，结果存在争议。到目前为止，对DSG 2在卵巢浆液性肿瘤（OST）中的表达及其与生存率和临床病理数据的关系知之甚少。本研究采用qRT-PCR和免疫组化方法分别检测了33例非固定标本和92例石蜡包埋OST标本（包括良性、交界性、低度和高度）中DSG 2的mRNA和蛋白表达。然后通过免疫组化检测162例高级别浆液性癌（HGSC）中DSG 2的表达，并将其表达水平与临床病理和预后数据相关。结果表明，DSG 2在良性肿瘤中的表达相对较弱，在细胞-细胞边界呈弱而完整的染色。这与正常输卵管上皮组织中的表达模式相似。DSG 2在交界性和恶性骨肉瘤中的表达趋势不一致。此外，我们发现DSG 2低表达与HGSC的预后差（P < 0.05）和高有丝分裂（P = 0.0042）相关。因此，DSG 2可能参与卵巢癌的进展，并在不同的OST中发挥不同的作用。此外，DSG 2的低表达与HGSC的不良预后相关。
Desmogleins (Dsgs) are major members of the desmosomal cadherins that are critically involved in cellcell adhesion and the maintenance of normal tissue architecture in epithelia. DSG2 is the most ubiquitous desmosomal cadherin; however, abnormal expression of DSG2 has been detected in several types of cancer with controversial results. So far, little is known about DSG2 expression in ovarian serous tumor (OST) and its associations with survival and clinicopathologic data. In this study, mRNA and protein expression of DSG2 was detected in 33 cases of nonfixed samples and 92 cases of paraffin-embedded OST specimens (including benign, borderline, low-grade, and high-grade) by using qRT-PCR and immunohistochemistry, respectively. DSG2 expression was then measured in 162 cases of high-grade serous carcinoma (HGSC) by immunohistochemistry, and the expression levels were correlated with clinicopathologic and prognostic data. As the results, DSG2 could be readily detected in benign tumor with relative weak expression at the mRNA level and showed weak but complete staining at the cell-cell borders. This was similar to the expression pattern in the normal fallopian epithelial tissue. However, the expression tendency of DSG2 at the mRNA and protein level was inconsistent in borderline and malignant OST. In addition, we found that a low DSG2 expression was associated with poor prognosis (P < 0.05) and high mitosis (P = 0.0042) of HGSC. Thus, DSG2 may be involved in the progression of ovarian cancer and plays a different role in different OST. Moreover, a low DSG2 expression was associated with poor prognosis of HGSC.