Exon skipping of AGAMOUS homolog PrseAG in developing double flowers of Prunus lannesiana (Rosaceae)
Exon skipping of AGAMOUS homolog PrseAG in developing double flowers of Prunus lannesiana (Rosaceae)
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AGAMOUS 同源物 PrseAG 的外显子跳跃在李属（蔷薇科）发育双花过程中的作用
DOI：
10.1007/s00299-012-1357-2
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发表时间：
2013-02-01
影响因子：
6.2
通讯作者：
Lu, Hai
中科院分区：
文献类型：
--
作者：
Liu, Zhixiong;Zhang, Dandan;Lu, Hai
拟南芥花同源异型C基因AGAMOUS（AG）分别与雄蕊和心皮同源异型。AG功能的丧失导致雄蕊向花瓣的同源异型转化和重瓣花的形成。为了对重瓣花的中国李（Prunus lannesiana，Rosaceae）进行分子解剖，我们从两个单瓣花和重瓣花的中国李中分离并鉴定了一个单拷贝基因AG同源物。序列分析表明，AG同源物，prseag-1，从双花显示了一个170 bp的外显子跳读相比，PrseAG（李serrulata AGAMOUS）从单花。基因组DNA序列分析表明，异常剪接导致突变的prseag-1蛋白与C-末端AG基序I和II缺失。此外，蛋白质序列比对和系统发育分析表明，PrseAG被归类为euAG谱系。半定量PCR分析表明，PrseAG基因仅在单花的雄蕊和心皮生殖器官中表达，而prseag-1基因在双花的花瓣、雄蕊和心皮中表达量较高。转35 S：：PrseAG基因的拟南芥极早开花，雄蕊和心皮变大，花瓣同源异型转化为雄蕊器官，但prseag-1的异位表达不能模拟PrseAG在拟南芥中的表型异位表达。总之，本研究提供了证据，证明重花'Albo-rosea'是一个假定的C功能ag突变体在拉氏杨。
Two transcript isoforms of AGAMOUS homologs, from single and double flower Prunus lannesiana, respectively, showed different functions.The Arabidopsis floral homeotic C function gene AGAMOUS (AG) confers stamen and carpel identity. Loss of AG function results in homeotic conversions of stamens into petals and formation of double flowers. In order to present a molecular dissection of a double-flower cultivar in Prunus lannesiana (Rosaceae), we isolated and identified a single-copy gene, AG homolog from two genetically cognate P. lannesiana bearing single and double flowers, respectively. Sequence analysis revealed that the AG homolog, prseag-1, from double flowers showed a 170-bp exon skipping as compared to PrseAG (Prunus serrulata AGAMOUS) from the single flowers. Genomic DNA sequence revealed that abnormal splicing resulted in mutant prseag-1 protein with the C-terminal AG motifs I and II deletions. In addition, protein sequence alignment and phylogenetic analyses revealed that the PrseAG was grouped into the euAG lineage. A semi-quantitative PCR analysis showed that the expression of PrseAG was restricted to reproductive organs of stamens and carpels in single flowers of P. lannesiana 'speciosa', while the prseag-1 mRNA was highly transcribed throughout the petals, stamens, and carpels in double flowers from 'Albo-rosea'. The transgenic Arabidopsis containing 35S::PrseAG displayed extremely early flowering, bigger stamens and carpels and homeotic conversion of petals into staminoid organs, but ectopic expression of prseag-1 could not mimic the phenotypic ectopic expression of PrseAG in Arabidopsis. In general, this study provides evidences to show that double flower 'Albo-rosea' is a putative C functional ag mutant in P. lannesiana.