Diffraction optical tomography using a quantitative phase imaging unit.
Diffraction optical tomography using a quantitative phase imaging unit.
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DOI：
10.1364/ol.39.006935
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发表时间：
2014-12-15
期刊：
影响因子：
3.6
通讯作者：
Park Y
中科院分区：
文献类型：
--
作者：
Kim K;Yaqoob Z;Lee K;Kang JW;Choi Y;Hosseini P;So PT;Park Y
提出了一种简单实用的测量生物细胞三维折射率分布的方法。在装有光束扫描装置的传统倒置显微镜上安装了一套由一套紧凑的偏振器组成的共光路自参考干涉仪，它可以在不同的照明角度下精确测量多个具有高相位稳定性的样品的二维全息图，由此可以再现样品的精确三维光学衍射层析图像。给出了聚苯乙烯微球等非生物样品以及包括人红细胞和乳腺癌细胞在内的生物样品的三维电阻抗断层图像。
A simple and practical method to measure three-dimensional (3-D) refractive index (RI) distributions of biological cells is presented. A common-path self-reference interferometry consisting of a compact set of polarizers is attached to a conventional inverted microscope equipped with a beam scanning unit, which can precisely measure multiple 2-D holograms of a sample with high phase stability for various illumination angles, from which accurate 3-D optical diffraction tomograms of the sample can be reconstructed. 3-D RI tomograms of nonbiological samples such as polystyrene microspheres, as well as biological samples including human red blood cells and breast cancer cells, are presented.