Antidepressant-like effects of paeoniflorin on post-stroke depression in a rat model
Antidepressant-like effects of paeoniflorin on post-stroke depression in a rat model
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芍药苷对大鼠模型中风后抑郁症的抗抑郁样作用
DOI：
10.1080/01616412.2019.1576361
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发表时间：
2019-05-04
影响因子：
1.9
通讯作者：
Liu, Bin
中科院分区：
文献类型：
--
作者：
Hu, Ming-zhe;Wang, An-rong;Liu, Bin
摘要背景：卒中后抑郁（PSD）是卒中后最常见的情绪障碍之一，通常导致不良结局。然而，PSD的潜在病理生理机制和有效的治疗方法仍不清楚。目的：研究芍药苷对PSD大鼠模型的抗抑郁作用。方法：将大鼠随机分为假手术对照组（Sham）、抑郁症组（PSD）、芍药苷组（PSD）和氟西汀组（PSD）。PSD是由右侧大脑中动脉闭塞后21天的慢性不可预测的轻度应激联合（CUMS）与单独升高引起的。采用蔗糖偏好实验和旷场实验评价其抑郁样行为。采用神经功能缺损评分和平衡木试验评价神经功能。采用免疫印迹和免疫荧光法检测海马复合体CA 1区磷酸化CREB（p-CREB）和脑源性神经营养因子（BDNF）的表达。结果：芍药苷和氟西汀治疗后抑郁样行为明显改善。此外，芍药苷治疗显着增加BDNF和p-CREB在CA 1区的表达。结论：结果表明，芍药苷能改善PSD大鼠的症状，提高其功能能力，其作用与氟西汀相似。缩略语：PSD：中风后抑郁;累计：慢性不可预测轻度应激刺激; MCAO：大脑中动脉闭塞; OFT：旷场试验; SPT：蔗糖偏好试验，NDS：神经功能缺损评分，BBT：平衡木平衡试验; BDNF：脑源性神经营养因子蛋白; p-CREB：磷酸化环磷酸腺苷反应元件结合蛋白
ABSTRACT Background: Post-stroke depression (PSD) is one of the most prevalent emotional disorders after stroke and often results in poor outcomes. However, the underlying physiopathologic mechanism and effective treatment of PSD remain poorly elucidated. Objective: To investigate whether paeoniflorin has antidepressant-like activity in a rat model of PSD. Methods: Rats were randomly divided into four groups: sham-operated control (Sham), PSD, paeoniflorin (with PSD) and fluoxetine group(with PSD). PSD was developed by the right middle cerebral artery occlusion followed 21 days chronic unpredictable mild stress combined (CUMS) with raised alone. Tests of sucrose preference and open field were used to assess the depression-like behavior. Neurological function was evaluated by neurological deficit score and beam balance test. Expression of phosphorylated CREB (p-CREB) and brain-derived neurotrophic factor (BDNF) in the CA1 region of the hippocampal complex was evaluated by western blot and immunofluorescence. Results: Te depressive-like behaviors markedly improved after paeoniflorin and fluoxetine treatment. Furthermore, paeoniflorin treatment significantly increased BDNF and p-CREB expression in the CA1 region. Conclusions: Observed results suggested that paeoniflorin could ameliorate the symptoms and improve the functional capability of PSD rats, similar to the effect of fluoxetine. Abbreviations: PSD: post-stroke depression; CUMS: chronic unpredictable mild stress stimulation; MCAO: middle cerebral artery occlusion; OFT: open field test; SPT: sucrose preference test, NDS: neurological deficit score, BBT: beam balance test; BDNF: brain-derived neurotrophic factor protein; p-CREB: phosphorylated Cyclic-AMP responsive element binding protein