Altered expression of Skp2, c-Myc and p27 proteins but not mRNA after H-pylori eradication in chronic gastritis
Altered expression of Skp2, c-Myc and p27 proteins but not mRNA after H-pylori eradication in chronic gastritis
复制标题
DOI：
10.1038/modpathol.3800476
复制
发表时间：
2006-01-01
期刊：
影响因子：
7.5
通讯作者：
Moss, SF
中科院分区：
文献类型：
--
作者：
Kim, SS;Meitner, P;Moss, SF
幽门螺杆菌感染与胃上皮细胞周转增加和非贲门胃癌有关。在Skp2介导的泛素化之后，细胞周期的进展依赖于p27的蛋白酶体降解，p27是一种周期蛋白依赖性激酶抑制剂和胃肿瘤抑制因子。c-Myc是p27的转录抑制因子，也是Skp2的靶标。在体外，幽门螺杆菌在翻译后降低p27蛋白。我们的目的是确定p27在体内是如何被幽门螺杆菌调节的。采用免疫组织化学和激光显微解剖技术，研究了根除幽门螺杆菌对22例慢性胃炎患者胃上皮细胞p27、Skp2和c-Myc蛋白及mRNA的影响。胃窦上皮细胞表达p27蛋白的比例在根除幽门螺杆菌后显著升高(平均+/- s.e.m.：根除前37 +/- 2.4% vs根除后55 +/- 2.8%
Helicobacter pylori infection is associated with increased gastric epithelial cell turnover and non-cardia gastric cancer. Cell cycle progression is dependent on the proteasomal degradation of p27, a cyclin-dependent kinase inhibitor and gastric tumor suppressor, following ubiquitination mediated by Skp2. c-Myc is a transcriptional repressor of p27 and also a target of Skp2. In vitro, H. pylori decreases p27 protein post-translationally. We aimed to determine how p27 is regulated by H. pylori in vivo. The effect of eradicating H. pylori on gastric epithelial p27, Skp2, and c-Myc proteins and mRNA was investigated in 22 patients with chronic gastritis, by immunohistochemistry and laser capture microdissection. The percentage of gastric antral epithelial cells expressing p27 protein was significantly higher after eradication of H. pylori (mean +/- s. e. m. 37 +/- 2.4% pre-eradication vs 55 +/- 2.8% post-eradication; P