Resolution improvement in optical projection tomography by the focal scanning method
Resolution improvement in optical projection tomography by the focal scanning method
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DOI：
10.1364/ol.35.003363
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发表时间：
2010-10-15
期刊：
影响因子：
3.6
通讯作者：
Seibel, Eric J.
中科院分区：
文献类型：
--
作者：
Miao, Qin;Hayenga, Jon;Seibel, Eric J.
光学投影层析成像（OPT）要求透镜的景深（DOF）覆盖至少一半的样品。在用高NA透镜获得高分辨率和用低NA透镜获得大DOF之间存在折衷。高NA物镜透镜的自由度可以通过扫描其焦平面穿过样品来扩展。我们将这种扩展的自由度图像称为“伪投影”。“从这些伪投影重建的图像具有等距分辨率，这可以与高NA物镜的横向分辨率相同。焦点扫描方法使OPT分辨率提高了10倍以上。(C)2010年美国光学学会
Optical projection tomography (OPT) requires the depth of field (DOF) of the lens to cover at least half of the sample. There is a trade-off between obtaining high resolution with a high-NA lens and obtaining large DOF with a low-NA lens. The DOF of a high-NA objective lens can be extended by scanning its focal plane through the sample. We call this extended DOF image a "pseudoprojection." Images reconstructed from these pseudoprojections have isometric resolution, which can be the same as the lateral resolution of the high-NA objective. The focal scanning method produces an over 10x improvement in OPT resolution. (C) 2010 Optical Society of America