Transcriptome and target DNA enrichment sequence data provide new insights into the phylogeny of vespid wasps (Hymenoptera: Aculeata: Vespidae)
Transcriptome and target DNA enrichment sequence data provide new insights into the phylogeny of vespid wasps (Hymenoptera: Aculeata: Vespidae)
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DOI：
10.1016/j.ympev.2017.08.020
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发表时间：
2017-11-01
影响因子：
4.1
通讯作者：
Niehuis, Oliver
中科院分区：
文献类型：
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作者：
Bank, Sarah;Sann, Manuela;Niehuis, Oliver
WASP家族Vespidae组成了5000多种描述的物种，这些物种代表了从孤立和社会主义到欧洲社会和社会寄生虫的生活历史策略。通过分析行为和形态学性状以及少数几个选定基因的核苷酸序列，已反复研究了主要Vespid WASP谱系（即亚家族和部落）的系统发育关系。在这里，我们使用系统生理学方法重建其系统发育关系。我们对24种Vespid黄蜂和8种外群物种的转录组进行了测序，并利用了转录序列，用于设计探针，以富集913个单拷贝蛋白质编码基因，以从其他25个乙醇固定的VESPID物种中用核苷酸序列数据补充转录组数据。对组合序列数据的系统发育分析的结果表明，Eusocial subfial stenogastrinae是所有剩余的vespidae的姐妹组，而eumeninae下属的Eumeninae被证明是副学的。在目前公认的Eumenine部落中，Odynerini相对于Eumenini是个销育的，而Zethini则相对于Polistinae和Vespinae是多个的。我们的结果与当前的Eumeninae部落细分相抵触，因此，我们建议将亚家族列为“ Zethini”的两个主要进化枝：Raphiglossinae和Zethinae。总体而言，我们的发现证实了Vespid Wasp中双层依恋性的两个独立起源的假设，并提出了使用Raphiglossinae，Zethinae，Polistinae和Vespesane使用咀嚼和垂涎的植物材料来建筑巢穴的单一起源。推断的系统发育关系和开放访问VESPID WASP靶DNA富集探针将为将来对Vespidae种类物种的比较研究提供宝贵的工具，包括其基因组，生活方式，社会性的进化以及与其他生物体的共同发展。
The wasp family Vespidae comprises more than 5000 described species which represent life history strategies ranging from solitary and presocial to eusocial and socially parasitic. The phylogenetic relationships of the major vespid wasp lineages (i.e., subfamilies and tribes) have been investigated repeatedly by analyzing behavioral and morphological traits as well as nucleotide sequences of few selected genes with largely incongruent results. Here we reconstruct their phylogenetic relationships using a phylogenomic approach. We sequenced the transcriptomes of 24 vespid wasp and eight outgroup species and exploited the transcript sequences for design of probes for enriching 913 single-copy protein-coding genes to complement the transcriptome data with nucleotide sequence data from additional 25 ethanol-preserved vespid species. Results from phylogenetic analyses of the combined sequence data revealed the eusocial subfamily Stenogastrinae to be the sister group of all remaining Vespidae, while the subfamily Eumeninae turned out to be paraphyletic. Of the three currently recognized eumenine tribes, Odynerini is paraphyletic with respect to Eumenini, and Zethini is paraphyletic with respect to Polistinae and Vespinae. Our results are in conflict with the current tribal subdivision of Eumeninae and thus, we suggest granting subfamily rank to the two major clades of "Zethini": Raphiglossinae and Zethinae. Overall, our findings corroborate the hypothesis of two independent origins of eusociality in vespid wasps and suggest a single origin of using masticated and salivated plant material for building nests by Raphiglossinae, Zethinae, Polistinae, and Vespinae. The inferred phylogenetic relationships and the open access vespid wasp target DNA enrichment probes will provide a valuable tool for future comparative studies on species of the family Vespidae, including their genomes, life styles, evolution of sociality, and co-evolution with other organisms.