Comparative transmission of bacteria from Artemia salina and Brachionus plicatilis to the cnidarian Nematostella vectensis.
Comparative transmission of bacteria from Artemia salina and Brachionus plicatilis to the cnidarian Nematostella vectensis.
复制标题
卤虫和褶皱臂尾轮虫向刺胞动物 Nematostella vectensis 的细菌传播比较。
DOI：
10.1093/femsec/fiac096
复制
发表时间：
2022
影响因子：
4.2
通讯作者：
Reitzel,AdamM
中科院分区：
文献类型：
--
作者：
Krueger,QuintonA;Shore,MadisunH;Reitzel,AdamM
与动物相关的微生物群落（微生物组）对宿主生物体的发育，生理和健康至关重要。了解微生物组组装的关键步骤是确定细菌如何有效地在宿主体内定植和建立。细菌通常通过垂直传播，被动地从环境中或通过食物消费来定殖宿主。采用饵料喂养法（PFM），以卤虫和褶皱臂尾轮虫为饵料，对Velezensis芽孢杆菌、螺旋假交替单胞菌和Vibrio alginolyticustoNematostella vectensis进行了透射率测试。我们比较PFM的溶液吸收法（SUM），以量化这些宿主生物体中的细菌浓度，平板计数。在6 h时，Larvastatin与SUM的摄取相似，但在48 h时浓度高于PFM。幼鱼在6 h时获得类似的浓度SUM和PFM usingB。plicatilisandA. salina。在第2天，从PFM载体的细菌数量增加。7天后，B组CFU减少2倍。plicatilisandA. salina相对于2d浓度，14d后进一步下降。因此，在24小时后，猎物介导的方法提供比SUM更大的微生物移植，支持这种方法作为单个细菌物种的更成功的接种方法。
The microbial community associated with animals (microbiome) is essential for development, physiology, and health of host organisms. A critical step to understand the assembly of microbiomes is to determine how effectively bacteria colonize and establish within the host. Bacteria commonly colonize hosts through vertical transmission, passively from the environment, or through food consumption. Using the prey feeding method (PFM), we test transmittance ofBacillus velezensis, Pseudoalteromonas spiralis, andVibrio alginolyticustoNematostella vectensisusing two prey,Artemia salinaandBrachionus plicatilis. We compare PFM to a solution uptake method (SUM) to quantify the concentration of bacteria in these host organisms, with plate counts. Larvae had a similar uptake with SUM at 6 h but had greater concentrations at 48 h versus PFM. Juveniles acquired similar concentrations at 6 h for SUM and PFM usingB. plicatilisandA. salina. At 2 days, the quantity of bacteria vectored from PFM increased. After 7 days the CFUs decreased 2-fold withB. plicatilisandA. salinarelative to the 2-day concentrations, and further decreased after 14 days. Therefore, prey-mediated methods provide greater microbe transplantation than SUM after 24 h, supporting this approach as a more successful inoculation method of individual bacterial species.