Platelet activation and induction of tissue factor in acute and chronic atrial fibrillation: Involvement of mononuclear cell-platelet interaction
Platelet activation and induction of tissue factor in acute and chronic atrial fibrillation: Involvement of mononuclear cell-platelet interaction
复制标题
DOI：
10.1016/j.thromres.2011.07.013
复制
发表时间：
2011-12-01
影响因子：
7.5
通讯作者：
Murohara, Toyoaki
中科院分区：
文献类型：
--
作者：
Hayashi, Mutsuharu;Takeshita, Kyosuke;Murohara, Toyoaki
背景：心房颤动（AF）与血栓前状态相关。本研究的目的是分析AF患者细胞-细胞相互作用下游的单核细胞（MNCs）和粒细胞中的血小板活化和组织因子（TF）诱导。在电生理学研究期间，在窦性心律和房颤诱导后15分钟从阵发性房颤患者（n = 14）中采集血样，对照组（n = 13）和门诊慢性房颤患者（n = 14）。用流式细胞术检测富血小板血浆（PRP）中血小板和微粒以及全血中单个核细胞和粒细胞上CD 41 a、CD 42 b、P-选择素和P-选择素糖蛋白配体-1（PSGL-1）的表达。结果：对照组和慢性AF组血小板和微粒表面CD 41 a和CD 42 b的表达水平相当，且在AF诱导后无变化。急性房颤诱导显著增加血小板和微粒上P-选择素的表达，并在类似程度上，P-选择素阳性的MNC和粒细胞和P-选择素/PSGL-1双阳性的MNC。然而，AF诱导对离体血小板-MNC相互作用或MNC和粒细胞上的TF表达没有影响。只有慢性AF患者表现出血小板-MNC相互作用离体和TF过表达MNCs.Conclusions：急性发作的AF激活血小板在几分钟内启动血小板-MNC相互作用。随后的血小板结合诱导TF表达的慢性AF患者。这些研究结果支持抗凝治疗慢性AF的疗效，并建议潜在的效用抗血小板治疗AF发生的早期阶段。(C)2011爱思唯尔有限公司保留所有权利。
Background: Atrial fibrillation (AF) is associated with a prothrombotic state. The aim of this study was to analyze platelet activation and tissue factor (TF) induction in mononuclear cells (MNCs) and granulocytes downstream of cell-cell interactions in AF patients.Methods: Blood samples were obtained from patients with paroxysmal AF (n = 14) at sinus rhythm and at 15 min after induction of AF during an electrophysiological study, and from control subjects (n = 13) and patients with chronic AF (n = 14) in the outpatient clinic. The expression of CD41a, CD42b, P-selectin, and P-selectin glycoprotein ligand-1 (PSGL-1) on platelets and microparticles in platelet-rich plasma (PRP), and on MNCs and granulocytes in whole blood were examined by flow cytometry. MNC-platelet interaction was investigated ex vivo.Results: The expression of CD41a and CD42b on platelets and microparticles was comparable between the control and chronic AF groups, and unchanged after AF induction. Acute induction of AF significantly increased the expression of P-selectin on platelets and microparticles, and to a similar extent, P-selectin-positive MNCs and granulocytes and P-selectin/PSGL-1-double positive MNCs. However, AF induction had no effect on platelet-MNC interactions ex vivo or TF expression on MNCs and granulocytes. Only patients with chronic AF showed platelet-MNC interaction ex vivo and TF overexpression on MNCs.Conclusions: Acute-onset AF activates platelets within minutes to initiate platelet-MNC interaction. The subsequent platelet binding induced TF expression in patients with chronic AF. These findings support the efficacy of anticoagulant therapeutics in chronic AF and suggest the underlying utility of antiplatelet therapeutics in early phase of AF occurrence. (C) 2011 Elsevier Ltd. All rights reserved.