Early pregnancy metabolite profiling discovers a potential biomarker for the subsequent development of gestational diabetes mellitus
Early pregnancy metabolite profiling discovers a potential biomarker for the subsequent development of gestational diabetes mellitus
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DOI：
10.1007/s00592-014-0626-7
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发表时间：
2014-10-01
影响因子：
3.8
通讯作者：
Baker, Philip N.
中科院分区：
文献类型：
--
作者：
de Seymour, Jamie V.;Conlon, Cathryn A.;Baker, Philip N.
目前用于确定有患妊娠期糖尿病风险的妇女的早期妊娠筛查工具缺乏特异性和敏感性。因此，胎儿和母亲在疾病发展过程中，在怀孕后期进行诊断和治疗之前，经常受到侮辱。代谢组学是一种分析方法，它允许评估生物体液中的小分子化合物。这项初步研究的目的是研究妊娠早期血清代谢产物与妊娠期糖尿病的关系，以寻找妊娠早期的生物标志物和潜在的代谢机制。我们的嵌套式病例对照研究分析了妊娠20周时的孕妇血清，这些血清来自新西兰妊娠终点筛查队列研究。代谢组谱用气相色谱和质谱联用进行，代谢物用R软件和内部质谱库鉴定。使用SPSS21.0版进行统计分析。在血清样品中鉴定出48种代谢物。与无并发症妊娠的对照组相比，随后患妊娠期糖尿病的妇女中衣康酸(P=0.0003)的含量显著增加，错误发现率为0.012。目前的初步研究发现衣康酸可能作为一种新的妊娠早期生物标志物来预测妊娠期糖尿病的后续发展。然而，这项先导性研究的结果在转化为临床环境之前需要在更大的、不同的人群中进行验证。
Current early pregnancy screening tools to identify women at risk of developing gestational diabetes mellitus lack both specificity and sensitivity. As a result, the foetus and mother are often subjected to insult during disease progression, prior to diagnosis and treatment in later pregnancy. Metabolomics is an analytical approach, which allows for appraisal of small molecular mass compounds in a biofluid. The aim of this pilot study was to investigate the relationship between the early gestation serum metabolite profile and the subsequent development of gestational diabetes mellitus in the search for early pregnancy biomarkers and potential metabolic mechanisms. Our nested case-control study analysed maternal serum at 20 weeks' gestation, obtained from the New Zealand cohort of the Screening for Pregnancy Endpoints study. Metabolomic profiling was performed using gas chromatography coupled to mass spectrometry, and metabolites were identified using R software and an in-house mass spectral library. Statistical analysis was performed using SPSS version 21.0. Forty-eight metabolites were identified in the serum samples. Itaconic acid (P = 0.0003), with a false discovery rate of 0.012, was found to be significantly more abundant in women who subsequently developed gestational diabetes mellitus, when compared to controls with uncomplicated pregnancies. The current pilot study found that itaconic acid may have potential as a novel biomarker in early pregnancy to predict the subsequent development of gestational diabetes mellitus. However, the findings from this pilot study require validation with a larger, diverse population before translation into the clinical setting.