Functional relationship between SpoVIF and GerE in gene regulation during sporulation of Bacillus subtilis.
Functional relationship between SpoVIF and GerE in gene regulation during sporulation of Bacillus subtilis.
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DOI：
10.1099/mic.0.26689-0
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发表时间：
2004
期刊：
影响因子：
1.5
通讯作者：
Ritsuko Kuwana;Hiromi Ikejiri;Satoko Yamamura;H. Takamatsu;K. Watabe
中科院分区：
文献类型：
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作者：
Ritsuko Kuwana;Hiromi Ikejiri;Satoko Yamamura;H. Takamatsu;K. Watabe
枯草芽孢杆菌产孢特异性SpoVIF(YjcC)蛋白对耐热芽胞的发育是必不可少的。Gere蛋白是LuxR-FixJ家族中最小的成员，包含一个螺旋-转角螺旋(HTH)基序，参与多种产孢特异性基因的表达。本研究分析了产孢子性spoVIF阴性细胞的基因表达和蛋白质组成。CgeA、CotG和Cots是依赖Gere的外壳蛋白，在spoVIF阴性的细胞中不表达。Northern杂交结果表明，SpoVIF对cgeA、cotG和cots转录的调控方式与Gere相似。在SpoVIF阴性细胞中，Gere mRNA转录正常，但抗Gere抗血清免疫印迹分析显示，Gere蛋白的量明显低于野生型对照。利用绿色荧光蛋白(GFP)融合蛋白，在荧光显微镜下观察SpoVIF和Gere的定位。SpoVIF-GFP和Gere-GFP在母细胞室中均可检测到。这些结果表明，SpoVIF直接或间接控制Gere蛋白的功能，在孢子形成的后期阶段，SpoVIF是基因调控所必需的。
The sporulation-specific SpoVIF (YjcC) protein of Bacillus subtilis is essential for the development of heat-resistant spores. The GerE protein, the smallest member of the LuxR-FixJ family, contains a helix-turn-helix (HTH) motif and is involved in the expression of various sporulation-specific genes. In this study, the gene expression and protein composition of sporulating spoVIF-negative cells were analysed. CgeA, CotG and CotS, which are GerE-dependent coat proteins, were not expressed in the spoVIF-negative cells. Northern blotting showed that SpoVIF regulated the transcription of cgeA, cotG and cotS in a manner similar to that of GerE. In spoVIF-negative cells, gerE mRNA was transcribed normally, but immunoblot analysis using anti-GerE antiserum showed that the quantity of GerE protein was considerably less than that in wild-type controls. Using GFP (green fluorescent protein) fusion proteins, the localization of SpoVIF and GerE was observed by fluorescence microscopy. SpoVIF-GFP was detectable in the mother cell compartment, as was GerE-GFP. These results suggest that SpoVIF directly or indirectly controls the function of the GerE protein, and that SpoVIF is required for gene regulation during the latter stages of sporulation.