Comprehensive molecular phylogeny of the sub-family Dipterocarpoideae (Dipterocarpaceae) based on chloroplast DNA sequences
Comprehensive molecular phylogeny of the sub-family Dipterocarpoideae (Dipterocarpaceae) based on chloroplast DNA sequences
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DOI：
10.1266/ggs.81.1
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发表时间：
2006-02-01
影响因子：
1.1
通讯作者：
Szmidt, Alfred E.
中科院分区：
文献类型：
--
作者：
Gamage, Dayananda Thawalama;de Silva, Morley P.;Szmidt, Alfred E.
龙脑香科是著名植物科龙脑香科中最大的亚科，在南亚雨林中占主导地位。尽管之前的几项研究探讨了龙脑香科的系统发育，但龙脑香亚科的许多属之间的关系仍不清楚。特别是，人们对 Vateriopsis、Stemonoporus、Vateria 属的关系知之甚少，并且 Shorea 和 Hopea 物种的系统发育结果和分类学分类之间仍然不一致。我们利用 trnL-trnF 间隔区、trnL 内含子和叶绿体 DNA (cpDNA) 的 matK 基因序列研究了龙脑香亚科的分子系统发育。这项研究是第一个基于cpDNA对龙脑香亚科进行全面的系统发育重建，因为它包括大多数属（14个）和大量物种（79个），其中大多数物种是斯里兰卡特有的，以及来自塞舌尔的一个物种和来自马达加斯加的Monotes属的一个物种。使用包括所有三个 cpDNA 区域的组合序列集，使用邻接 (NJ) 和最大似然 (ML) 方法构建系统发育树。 NJ和ML树的拓扑结构在一定程度上与当前基于形态学的龙脑香科分类学以及之前基于cpDNA的分子系统发育一致。此外，我们的结果提供了关于以下属的关系的新证据：Vateriopsis 和 Stemonoporus，以及关于先前基于形态学的 Shorea 物种分类的有效性。此外，我们的树的拓扑结构与 Maury (1978)、Maury-Lechon (1979) 和 Symington (1943) 提出的 Shorea 物种分类一致。最后，我们的结果为Monotes属与亚洲Dipterocarpoideae而不是Tiliaceae的亲和力提供了证据，并表明它是前亚科未来研究的外群物种的良好候选者。
Dipterocarpoideae, the largest sub-family of well-known plant family Dipterocarpaceae, dominates in South Asian rain forests. Although several previous studies addressed the phylogeny of the Dipterocarpaceae family, relationships among many of its genera from the Dipterocarpoideae sub-family are still not well understood. In particular, little is known about the relationships of the genera Vateriopsis, Stemonoporus, Vateria and inconsistence remains between phylogenetic results and taxonomic classifications of Shorea and Hopea species. We studied molecular phylogeny of the sub-family Dipterocarpoideae using the trnL-trnF spacer, trnL intron and the matK gene sequences of chloroplast DNA (cpDNA). This study is the first comprehensive phylogeny reconstruction for the sub-family Dipterocarpoideae based on cpDNA, as it includes most genera (14) and a large number of species (79) with most species endemic to Sri Lanka, as well as one species from Seychelles and one species from the genus Monotes from Madagascar. Phylogenetic trees were constructed using the Neighbor Joining (NJ) and Maximum Likelihood (ML) methods using combined set of sequences including all three cpDNA regions. The topologies of the NJ and ML trees were to a certain extent, consistent with the current taxonomy of Dipterocarpoideae based on morphology and with previous molecular phylogenies based on cpDNA. Furthermore, our results provided new evidence regarding the relationships of the following genera: Vateriopsis and Stemonoporus and about the validity of the previous morphology based classifications of Shorea species. In addition, the topology of our trees was consistent with the classification of Shorea species proposed by Maury (1978), Maury-Lechon (1979) and Symington (1943). Finally, our results provided evidence for the affinity of the genus Monotes to Asian Dipterocarpoideae rather than to Tiliaceae and indicated that it is a good candidate for outgroup species for future studies of the former sub-family.