An Investigation to Optimize Angiogenesis within Potential Dermal Replacements
An Investigation to Optimize Angiogenesis within Potential Dermal Replacements
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优化潜在真皮替代物中血管生成的研究
DOI：
10.1097/01.prs.0000218843.86011.f8
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发表时间：
2006
影响因子：
3.6
通讯作者：
R. Sanders
中科院分区：
文献类型：
--
作者：
M. Potter;C. Linge;P. Cussons;J. Dye;R. Sanders
背景：急性和慢性创伤费用高昂，而且总是暴露出重要的结构。外科重建会导致供体部位的发病率、疤痕形成和需要重症监护。使用人造真皮重建避免了供体部位，但现有的胶原蛋白溶液容易受到不良影响。使用体外血管生成试验，作者研究了第二代促血管生成合成真皮替代物中真皮基质的潜在包含物。方法：人胎盘内皮细胞与Cytodex微珠(Pharmacia Biotech)共培养，加入8种不同的细胞外基质凝胶(胶原、纤维蛋白、4种糖胺多聚糖、玻璃体连接蛋白和纤维连接蛋白)，加或不加两种可溶性血管生成因子刺激。使用了三种不同的细胞系，每种条件下有30个珠子。用Sigma Scan计算机软件计算细胞对凝胶的侵袭能力，并进行统计学比较。结果：作者发现纤维蛋白对内皮细胞的侵袭提供了最大的刺激，而胶原以浓度依赖的方式抑制了纤维蛋白的侵袭。替代性细胞外基质成分和可溶性血管生成因子的侵袭力远低于纤维蛋白。结论：与可溶性血管生成因子相比，细胞外基质可提供更大的血管生成潜能。纤维蛋白提供了比胶原更好的促血管生成支架。这很可能被用来鼓励血管进入，并最终接受第二代促血管生成的合成真皮替代物。
Background: Acute and chronic wounds are costly and invariably expose significant structures. Surgical reconstruction causes donor-site morbidity, scarring, and the need for intensive care. Reconstruction using an artificial dermis avoids donor sites, but available collagen-based solutions are susceptible to poor take. Using an in vitro angiogenic assay, the authors investigated dermal matrices for potential inclusion in a second-generation proangiogenic synthetic dermal replacement. Methods: Human placental endothelial cells were cocultured on Cytodex beads (Pharmacia Biotech) and plated in eight different extracellular matrix gels (collagen, fibrin, four glycosaminoglycans, vitronectin, and fibronectin), with or without stimulation from two soluble angiogenic factors. Three different cell lines were used, with 30 beads per condition. Cellular invasion into gels was calculated using Sigma Scan computer software, and statistical comparisons were made. Results: The authors found that fibrin provided greatest stimulus for endothelial invasion, with invasion in fibrin inhibited by collagen in a concentration-dependent fashion. Invasion by alternative extracellular matrix components and soluble angiogenic factors was far less than that in fibrin. Conclusions: The authors identified that extracellular matrices can provide greater angiogenic potential than soluble angiogenic factors. Fibrin provided a better proangiogenic scaffold than collagen. This could well be used to encourage blood vessel ingress and eventual take of a second-generation proangiogenic synthetic dermal replacement.
影响因子：
3.1
作者：
Nakatsu, MN;Sainson, RCA;Hughes, CCW
通讯作者：
Hughes, CCW
影响因子：
3.3
作者：
Sottile, J;Hocking, DC
通讯作者：
Hocking, DC