The Effect of Growth Hormone on Lipid Accumulation or Maturation in Adipocytes
The Effect of Growth Hormone on Lipid Accumulation or Maturation in Adipocytes
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生长激素对脂肪细胞脂质积累或成熟的影响
DOI：
10.1159/000447909
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发表时间：
2016-01-01
影响因子：
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通讯作者：
Wang, Xiangdong
中科院分区：
文献类型：
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作者：
Zhang, Yuchao;Yu, Hongsheng;Wang, Xiangdong
背景：脂肪细胞的成脂过程包括起始和成熟两个阶段。生长激素（GH）的分泌减少，在肥胖的受试者和GH水平呈负相关，腹部脂肪量。生长激素（GH）对脂肪细胞脂质积累和成熟的影响至今仍不清楚。方法：采用体内外实验研究生长激素对脂质蓄积的影响。采用cDNA微阵列、定量真实的时间PCR（qPCR）和蛋白质印迹法分析GH处理的3 T3-F442 A前体和成熟脂肪细胞以及GH处理的C57 BL/6小鼠附睾脂肪组织中脂肪细胞脂质积累或降解相关基因的表达。酶联免疫吸附法测定F442 A脂肪细胞培养上清中脂联素的水平。结果如下：结果发现，在3 T3-F442 A细胞中，尤其是在成脂启动后6d，GH干预导致脂肪细胞成熟调节因子（C/EBPα、PPARγ）和与脂质合成相关的主要基因（FAS、FABP）的表达降低，而UCP 1的表达显著增强。cDNA微阵列分析和qPCR结果显示，GH处理后3 T3-F442 A成熟脂肪细胞中SOCS 2和Adipor 2的表达增加。GH处理可增加成熟脂肪细胞脂联素和UCP 1的mRNA表达。上述结果通过体内研究得到证实。结论：生长激素可能负性调节脂肪细胞的成熟和脂质积累。
Background: Adipogenesis of adipocytes includes two stages: initiation and maturation. Growth hormone (GH) secretion is decreased in obese subjects and GH levels are inversely correlated with abdominal fat mass. The effects of growth hormone (GH) on lipids accumulation or maturation of adipocytes remains elusive. Methods: In the present study, effect of GH on lipid accumulation in vitro and in vivo was examined. cDNA microarray, quantitative real time-PCR (qPCR) and western blotting was used to analyze the expression of genes related to adipocyte lipid accumulation or degradation in pre- or mature 3T3-F442A adipocytes treated with GH and in epididymal adipose tissue of C57BL/6 mice administrated with GH. Level of adiponectin in supernatants of cultured F442A adipocytes was determined by enzyme-linked immune-sorbent assay. Results: We found that in 3T3-F442A especially 6 days post initiation of adipogenesis, GH intervention resulted in decreased expression of adipocyte maturation regulators (C/EBPα, PPARγ) and prominent genes related to lipid synthesis such as FAS and FABP, while the expression of UCP1 was markedly enhanced. cDNA microarray analysis and qPCR showed that the expression of SOCS2 and Adipor2 was increased under GH-treatment in mature 3T3-F442A adipocytes. GH treatment increased the mRNA expression of adiponectin and UCP1 in mature adipocytes. The above results were confirmed by in vivo study. Conclusions: GH potentially negatively modulates the maturation and accumulation of lipid in adipocytes.