Embryonic stem cells biosensor and its application in drug analysis and toxin detection
Embryonic stem cells biosensor and its application in drug analysis and toxin detection
复制标题
胚胎干细胞生物传感器及其在药物分析和毒素检测中的应用
DOI：
10.1109/jsen.2007.908439
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发表时间：
2007-11-01
影响因子：
4.3
通讯作者：
Wang, Ping
中科院分区：
文献类型：
--
作者：
Liu, Qingjun;Cai, Hua;Wang, Ping
为了研究干细胞作为生物传感器元件的用途，开发了一种新型的基于细胞的光可寻址电位传感器（LAPS）来监测细胞跳动。小鼠胚胎干细胞在体外被诱导分化为心肌细胞。通过LAPS记录干细胞诱导的自发跳动心肌细胞的细胞外场电位，电位和频率范围分别为25-45μV和0.5-3Hz。由于其能够在体外监测电位、频率等重要生理参数，该传感器可用于长期、无创的药物分析和毒素检测。药理学和毒理学研究使得使用基于干细胞的生物传感器进行生物医学检测成为可能。
To investigate the use of stem cells as biosensor elements, a novel cell-based light-addressable potentiometric sensor (LAPS) was developed for monitoring cellular beating. Mouse embryonic stem cells were induced to differentiate into cardiomyocytes in vitro. Extracellular field potentials of spontaneously beating cardiomyocytes induced from stem cells were recorded by LAPS in the potential and frequency ranges of 25-45 mu V and 0.5-3 Hz, respectively. Due to its capability of monitoring important physiological parameters such as potential and frequency in vitro, the sensor can be used in drug analysis and toxin detection in a long-term and noninvasive way. The pharmacological and toxicological researches make it possible to use stem cells-based biosensor for biomedical assays.