Cloning and Analysis of Promoter Regions of Flavonoid Biosynthesis Genes in Safflower
Cloning and Analysis of Promoter Regions of Flavonoid Biosynthesis Genes in Safflower
复制标题
红花类黄酮生物合成基因启动子区的克隆及分析
DOI：
10.1007/s11105-018-1070-6
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发表时间：
2018-03
影响因子：
2.1
通讯作者：
裴瑾
中科院分区：
文献类型：
--
作者：
任超翔;唐小慧;陈江;吴沂芸;吴清华;王黎;王倩;裴瑾
红花属植物Carthamus tinctoriusL.由于其能够改善脑血流量，已被广泛应用于传统中药中。黄酮类化合物是红花植物的主要活性成分。分析黄酮类化合物的调控对于人工调控其生物合成具有重要意义，以往的研究主要集中在红花黄酮类化合物的代谢合成方面。为了更好地了解红花黄酮类化合物生物合成的调控机制，本研究对红花黄酮类化合物生物合成基因启动子进行了克隆和分析。首先，分析了红花类黄酮基因的表达。24个参与类黄酮生物合成的基因表现出很大的差异。克隆并分析了4个主要在红花花中表达的类黄酮基因启动子（pctCHI-1、pctCHS-2、pctF 3 H-2和pctGT-1）。识别了响应于光的元素，即植物激素和MYB调节剂。使用原生质体系统分析启动子活性。4种启动子在花原生质体中都有活性，其中pctCHS-2的活性最高。我们的研究结果还表明pctCHS-2是一个花特异性启动子。这些结果为进一步研究红花中黄酮类化合物的调控作用提供了依据。
The flower of the safflower plantCarthamus tinctoriusL. has been widely used in traditional Chinese medicine because of its ability to improve cerebral blood flow. Flavonoids are the main active ingredient of safflower plants. Analyses of the regulation of flavonoids are of great significance for the artificial regulation of their biosynthesis, and previous studies have mainly focused on their metabolic synthesis in safflower. For a better understanding of the regulation of flavonoid biosynthesis in safflower, promoters of flavonoid biosynthesis genes in safflower were cloned and analysed. First, the expression of the flavonoid genes in safflower was analysed. Twenty-four genes involved in flavonoid biosynthesis exhibited large differences. Four promoters of flavonoid genes that were mainly expressed in the flowers of safflower (termedpctCHI-1,pctCHS-2,pctF3H-2andpctGT-1) were cloned and analysed. Elements that respond to light, i.e. phytohormones and the MYB regulator, were identified. The promoter activities were analysed using a protoplast system. All four promoters had activities in the flower protoplast, and thepctCHS-2activity was the highest. Our results also demonstrate thatpctCHS-2is a flower-specific promoter. All of these results provide a basis for improved analyses of flavonoid regulation in safflower.
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影响因子：
2.1
作者：
Xiao-Ping Fang;D. Kang;Chengjian Xie;Xingyong Yang;Anping Sui
通讯作者：
Xiao-Ping Fang;D. Kang;Chengjian Xie;Xingyong Yang;Anping Sui
影响因子：
9.5
作者：
B. Weisshaar;G. Jenkins
通讯作者：
B. Weisshaar;G. Jenkins
用于瞬时基因表达和研究程序性细胞死亡相关过程的高效玉米珠心原生质体系统
影响因子：
6.2
作者：
Chen, Jiang;Yi, Qiang;Huang, Yubi
通讯作者：
Huang, Yubi
影响因子：
6.9
作者：
Chen, Jiang;Yi, Qiang;Huang, Yubi
通讯作者：
Huang, Yubi
影响因子：
3.7
作者：
Lulin H;Xiao Y;Pei S;Wen T;Shangqin H
通讯作者：
Shangqin H