Label-free colorimetric sensor for ultrasensitive detection of heparin based on color quenching of gold nanorods by graphene oxide
Label-free colorimetric sensor for ultrasensitive detection of heparin based on color quenching of gold nanorods by graphene oxide
复制标题
基于氧化石墨烯金纳米棒颜色淬灭的无标记比色传感器，用于超灵敏检测肝素
DOI：
10.1016/j.bios.2012.02.008
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发表时间：
2012-04-15
影响因子：
12.6
通讯作者：
Wang, Wenhai
中科院分区：
文献类型：
--
作者：
Fu, Xiuli;Chen, Lingxin;Wang, Wenhai
利用氧化石墨烯（GO）的超强颜色猝灭能力，建立了一种新的无标记比色法用于肝素的超灵敏检测。十六烷基三甲基溴化铵（CTAB）稳定的金纳米棒（AuNRs）可以通过静电相互作用轻易地自组装到GO表面，导致表面等离子体共振（SPR）吸收降低，从而导致AuNRs从深到浅的颜色猝灭变化。聚阳离子鱼精蛋白被用作干扰AuNR和GO之间的静电相互作用的介质。由于鱼精蛋白和GO之间的相互作用较强，因此阻止了AuNRs被吸附到GO表面上，显示出AuNRs的天然颜色。而肝素更容易与鱼精蛋白联合收割机结合，在肝素存在下，金纳米粒子可以自组装到GO表面，导致金纳米粒子的天然颜色消失。随着肝素浓度的增加，AuNRs的颜色会逐渐变淡，直到几乎无色。自组装金核受体的量与肝素的浓度成正比，因此SPR吸收和颜色的变化被用来监测肝素水平。在优化条件下，该方法在0.02-0.28 μ g/mL范围内线性关系良好（R = 0.9957），检测限为5 ng/mL。同时拥有高灵敏度和选择性，简单，快速，和可视化使该传感器是潜在的适用于超灵敏和快速的现场检测对微量肝素。(c)2012爱思唯尔有限公司版权所有。
A novel label-free colorimetric strategy was developed for ultrasensitive detection of heparin by using the super color quenching capacity of graphene oxide (GO). Hexadecyltrimethylammonium bromide (CTAB)-stabilized gold nanorods (AuNRs) could easily self-assembly onto the surface of GO through electrostatic interaction, resulting in decrease of the surface plasmon resonance (SPR) absorption and consequent color quenching change of the AuNRs from deep to light. Polycationic protamine was used as a medium for disturbing the electrostatic interaction between AuNRs and GO. The AuNRs were prevented from being adsorbed onto the surface of GO because of the stronger interaction between protamine and GO, showing a native color of the AuNRs. On the contrary, in the presence of heparin, which was more easily to combine with protamine, the AuNRs could self-assembly onto the surface of GO, resulting in the native color disappearing of AuNRs. As the concentration of heparin increased, the color of AuNRs would gradually fade until almost colorless. The amounts of self-assembly AuNRs were proportional to the concentration of heparin, and thereby the changes in the SPR absorption and color had been used to monitor heparin levels. Under optimized conditions, good linearity was obtained in a range of 0.02-0.28 mu g/mL (R = 0.9957), and a limit of detection was 5 ng/mL. The simultaneous possession of high sensitivity and selectivity, simplicity, rapidity, and visualization enabled this sensor to be potentially applicable for ultrasensitive and rapid on-site detection toward trace heparin. (c) 2012 Elsevier B.V. All rights reserved.