Concomitant enlargement of perivascular spaces and decrease in glymphatic transport in an animal model of cerebral small vessel disease
Concomitant enlargement of perivascular spaces and decrease in glymphatic transport in an animal model of cerebral small vessel disease
复制标题
脑小血管疾病动物模型中血管周围空间的同时扩大和类淋巴运输的减少
DOI：
10.1016/j.brainresbull.2020.04.008
复制
发表时间：
2020-08-01
影响因子：
3.8
通讯作者：
Fu, Jianhui
中科院分区：
文献类型：
--
作者：
Xue, Yang;Liu, Na;Fu, Jianhui
目的：目的：观察自发性高血压大鼠（SHR）的胶质淋巴转运功能，评价血管周围间隙（PVS）扩大的程度。方法：以SHR为脑小血管病动物模型，以Wistar京都（WKY）大鼠为对照组。组织学检查评价室间隔扩大情况。将荧光示踪剂注入大鼠的小脑延髓池，随后定量暴露于荧光示踪剂的脑实质面积的比例，以评估胶质淋巴系统的流入和流出功能。结果：与WKY大鼠相比，SHR大鼠PVS明显增大，胶质淋巴系统的内流和外流功能明显降低。结论：胶质淋巴转运障碍可能参与了脑小血管病的发病过程，PVS增大可能是胶质淋巴系统受损的表现。
Objectives: To observe glymphatic transport and evaluate enlarged perivascular spaces (PVSs) in spontaneously hypertensive rats (SHRs).Methods: SHRs were used as an animal model of cerebral small vessel disease, and Wistar Kyoto (WKY) rats were used as the control group. Histopathology was used to evaluate the enlargement of PVSs. A fluorescent tracer was infused into the cisterna magna of rats, and the proportion of the brain parenchyma area exposed to the fluorescent tracer was later quantified to evaluate the influx and efflux function of the glymphatic system. The global and polarized expression of aquaporin protein 4 (AQP4) was analyzed by immunofluorescence.Results: Compared with WKY rats, SHRs exhibited obviously enlarged PVSs and significantly decreased influx and efflux function of the glymphatic system. The results showed a significant decrease in AQP4 polarity in SHRs, but a difference in global AQP4 expression was not observed between SHRs and WKY rats.Conclusions: Impaired glymphatic transport may be involved in the pathogenesis of arteriolosclerotic cerebral small vessel disease, and enlarged PVSs may be a manifestation of the impaired glymphatic system.