Metabolomic analysis of thermally injured and/or septic rats
Metabolomic analysis of thermally injured and/or septic rats
复制标题
热损伤和/或败血症大鼠的代谢组学分析。
DOI：
10.1016/j.burns.2010.03.015
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发表时间：
2010-11-01
期刊：
影响因子：
2.7
通讯作者：
Zhang, Wei-Dong
中科院分区：
文献类型：
--
作者：
Liu, Xin-Ru;Zheng, Xing-Feng;Zhang, Wei-Dong
Introduction: Early diagnosis and treatment for thermal injury with septic complications continue to be a serious clinical problem. In this study, plasma biomarkers of rats in the burn and/or septic models were investigated with a metabolomic method.Methods: Rat plasma samples were analyzed by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPL-CQ-TOF-MS). Multivariate analysis, the principal components analysis (PCA), was used to validate metabolic changes. In addition, another multivariate method, the orthogonal partial least-squares analysis (OPLS), was used to profile potential biomarkers in models.Results: Nine characteristic metabolites, including hypoxanthine, indoxyl sufate, glucuronic acid, gluconic acid, proline, uracil, nitrotyrosine, uric acid, and trihydroxy cholanoic acid were identified in models of thermal injury and/or sepsis.Conclusion: These biomarkers were mainly involved in oxidative stress and tissue damage, and might supply evidence for distinguishing burned septic patients from non-septic ones. (C) 2010 Elsevier Ltd and ISBI. All rights reserved.
