Genes involved in cell cycle G1 checkpoint control are frequently mutated in human melanoma metastases.
Genes involved in cell cycle G1 checkpoint control are frequently mutated in human melanoma metastases.
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DOI：
10.1038/bjc.1996.460
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发表时间：
1996-09
影响因子：
8.8
通讯作者：
Ringborg, U
中科院分区：
文献类型：
--
作者：
Platz, A;Sevigny, P;Norberg, T;Ring, P;Lagerlof, B;Ringborg, U
关键词：
癌细胞的一个共同特征是不受限制的细胞分裂。这可能是由编码细胞周期控制网络成分的基因突变引起的。参与G1检查点控制的基因的改变已经在许多人类肿瘤中登记，来自几个实验室的研究表明，这些改变加在一起是癌细胞中检测到的最常见的变化。本文应用聚合酶链反应-单链构象多态性（PCR/SSCP）技术对25例恶性黑色素瘤患者的26个转移瘤进行了p15、p53和N-ras基因突变分析和核苷酸序列分析。登记的突变频率与相同患者样本中先前登记的p16突变加在一起，使至少一种研究基因突变的患者的总频率达到44%。这些结果显示在人黑素瘤肿瘤样品收集物中细胞周期控制机制的组分之间存在异质缺陷，并证明检测到的改变的总频率随着筛选组中包括的细胞周期控制基因的数量而增加。
A common characteristic of cancer cells is unrestrained cell division. This may be caused by mutational changes in genes coding for components of cell cycle-controlling networks. Alterations in genes involved in G1 checkpoint control have been registered in many human tumours, and investigations from several laboratories show that such alterations, taken together, are the most frequent changes detected in cancer cells. The present paper describes mutational analysis by polymerase chain reaction-single-strand conformation polymorphism (PCR/SSCP) and nucleotide sequence analysis of the genes coding for the p15, p53 and N-ras proteins in 26 metastases from 25 melanoma patients. The registered mutation frequencies add together with previously registered mutations in p16 in the same patient samples to a substantial total frequency of 44% of patients with mutation in at least one of the investigated genes. These results show the occurrence of heterogeneous defects among components of the cell cycle controlling machinery in a human melanoma tumour sample collection and demonstrate that the total frequency of detected alterations increases with the number of cell cycle controlling genes included in the screening panel.
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DOI：
10.1073/pnas.91.6.2245
发表时间：
1994-03-15
影响因子：
11.1
作者：
LAGERKVIST, A;STEWART, J;LANDEGREN, U
通讯作者：
LANDEGREN, U
影响因子：
64.8
作者：
HANNON, GJ;BEACH, D
通讯作者：
BEACH, D
影响因子：
56.9
作者：
HOLLSTEIN, M;SIDRANSKY, D;HARRIS, CC
通讯作者：
HARRIS, CC
影响因子：
30.8
作者：
KAMB, A;SHATTUCKEIDENS, D;CANNONALBRIGHT, LA
通讯作者：
CANNONALBRIGHT, LA
影响因子：
56.9
作者：
CANNONALBRIGHT, LA;GOLDGAR, DE;SKOLNICK, MH
通讯作者：
SKOLNICK, MH