Proliferating endothelial cells, but not microvessel density, are a prognostic parameter in human cutaneous melanoma
Proliferating endothelial cells, but not microvessel density, are a prognostic parameter in human cutaneous melanoma
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DOI：
10.1097/01.cmr.0000232291.68666.4c
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发表时间：
2006-10-01
影响因子：
2.2
通讯作者：
Griffioen, Arjan W.
中科院分区：
文献类型：
--
作者：
Hillen, Femke;van de Winkel, Anouk;Griffioen, Arjan W.
血管生成的诱导在大多数人类肿瘤的发展中是至关重要的。血管生成通常通过肿瘤微血管的密度来评估。这项技术揭示了有争议的结果，在黑色素瘤血管生成的临床和预后价值。我们研究了58例皮肤黑色素瘤患者的肿瘤组织中的血管生成，其中临床随访超过10年，通过评估微血管密度和计数增殖内皮细胞的数量。为此，通过CD 31/CD 34染色对血管进行免疫化学检测，并用增殖标记物Ki 67对增殖的内皮细胞进行双重染色计数。我们发现微血管密度与肿瘤分期或存活率无关，无论是在瘤内还是瘤周区域。相反，增殖的内皮细胞仅在肿瘤内区域观察到，并且与肿瘤分期和远处转移的存在呈正相关。此外，发现与增殖的肿瘤细胞的数量呈强正相关。最后，大量生长的内皮细胞预示着短的生存期。我们的研究结果表明，血管生成可以最好地测量增殖内皮细胞计数，这个参数在皮肤黑色素瘤患者的预后价值。
The induction of angiogenesis is crucial in the development of most human tumors. Angiogenesis is routinely assessed by the density of tumor microvessels. This technique reveals controversial results on the clinical and prognostic value of angiogenesis in melanoma. We investigated angiogenesis in tumor tissues of 58 cutaneous melanoma patients, of which a clinical follow-up of over 10 years was available, through assessment of microvessel density and by enumeration of the number of proliferating endothelial cells. To that end, vessels were immunohistochemically detected by CD31/CD34 staining, and proliferating endothelial cells were enumerated in a double staining with the proliferation marker Ki67. We found that microvessel density did not correlate with tumor stage or survival, neither in intratumoral nor in peritumoral areas. In contrast, proliferating endothelial cells were only observed in intratumoral areas and were correlated positively with tumor stage and the presence of distant metastases. In addition, a strong positive correlation was found with the number of proliferating tumor cells. Finally, high numbers of growing endothelial cells predicted short survival. Our results show that angiogenesis could best be measured by enumeration of proliferating endothelial cells and that this parameter has prognostic value in patients with cutaneous melanoma.