Interphase death and reproductive death in X-irradiated MOLT-4 cells.
Interphase death and reproductive death in X-irradiated MOLT-4 cells.
复制标题
X 射线照射的 MOLT-4 细胞的间期死亡和生殖死亡。
作者：
K. Shinohara;H. Nakano
采用集落形成试验、染料排斥试验和细胞生长分析研究了电离辐射对MOLT-4细胞的杀伤作用。MOLT-4细胞经X射线照射后，在37 ℃孵育过程中，赤藓红B染色的细胞比例明显增加，24 h内达到最大值。相比之下，对于L5178 Y细胞或其衍生物、放射敏感性突变体M10细胞，在导致与通过殖民地形成测定测量的细胞存活水平相同的剂量下，未观察到这种增加。在MOLT-4细胞中，通过染料排除试验测量的细胞存活与通过殖民地形成试验测量的细胞存活进行比较的详细分析表明，MOLT-4细胞经历间期死亡以及生殖死亡。使用染料排除试验，亚克隆分析显示染色细胞水平存在各种差异。结果证实了MOLT-4细胞的群体异质性。此外，当用两种测定法测量时，没有一个亚克隆显示出完全相同的细胞存活率。目前的结果强烈表明，这两种模式的细胞死亡可能发生在单个MOLT-4细胞。细胞凋亡可能是这些细胞中两种死亡模式的机制。
The killing effects of ionizing radiation on MOLT-4 cells were studied using colony formation assays, dye exclusion tests, and cell growth analysis. When MOLT-4 cells were exposed to X rays, the fraction of cells stained with erythrosin B increased markedly during the incubation after irradiation at 37 degrees C and reached a maximum within 24 h. In contrast, no such increase was observed for L5178Y cells or their derivative, radiosensitive mutant M10 cells, at a dose resulting in the same level of cell survival as measured by the colony formation assay. Detailed analysis of cell survival as measured by the dye exclusion test in comparison with that measured by the colony formation assay in MOLT-4 cells indicated that MOLT-4 cells are subject to interphase death as well as reproductive death. Analysis of subclones showed a wide variety of differences in the level of stained cells using the dye exclusion test. The results confirmed the population heterogeneity of MOLT-4 cells. Moreover, none of the subclones showed exactly the same cell survival when this was measured with the two assays. The present results strongly suggest that both modes of cell death may occur in single MOLT-4 cells. Possibly apoptosis is the mechanism accounting for both modes of death in these cells.
影响因子：
5.8
作者：
BARRY, MA;BEHNKE, CA;EASTMAN, A
通讯作者：
EASTMAN, A