Fusarium oxysporum as a multihost model for the genetic dissection of fungal virulence in plants and mammals
Fusarium oxysporum as a multihost model for the genetic dissection of fungal virulence in plants and mammals
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DOI：
10.1128/iai.72.3.1760-1766.2004
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发表时间：
2004-03-01
影响因子：
3.1
通讯作者：
Di Pietro, A
中科院分区：
文献类型：
--
作者：
Ortoneda, M;Guarro, J;Di Pietro, A
真菌病原体在植物和动物宿主中引起疾病。这两类宿主之间感染机制的保守程度尚不清楚。我们提出了一种基于单株尖孢镰刀菌的植物-动物双重感染系统，尖孢镰刀菌是植物维管萎缩病的病原体，也是一种新出现的人类机会性病原体。将番茄致病分离物4287的微孢子注入免疫抑制小鼠的尾侧静脉，可引起多器官播散性感染和动物死亡。在小鼠模型中测试了编码丝裂原激活蛋白激酶、pH反应转录因子或V类几丁质合成酶的基因的敲除突变，这些基因以前被证明与番茄植物的毒力有关。结果表明，其中一些毒力因子在番茄植株和小鼠的侵染过程中起着不同的功能作用。因此，单株尖孢镰刀菌可用于研究植物和哺乳动物病程中的真菌毒力机制。
Fungal pathogens cause disease in plant and animal hosts. The extent to which infection mechanisms are conserved between both classes of hosts is unknown. We present a dual plant-animal infection system based on a single strain of Fusarium oxysporum, the causal agent of vascular wilt disease in plants and an emerging opportunistic human pathogen. Injection of microconidia of a well-characterized tomato pathogenic isolate (isolate 4287) into the lateral tail vein of immunodepressed mice resulted in disseminated infection of multiple organs and death of the animals. Knockout mutants in genes encoding a mitogen-activated protein kinase, a pH response transcription factor, or a class V chitin synthase previously shown to be implicated in virulence on tomato plants were tested in the mouse model. The results indicate that some of these virulence factors play functionally distinct roles during the infection of tomato plants and mice. Thus, a single F. oxysporum strain can be used to study fungal virulence mechanisms in plant and mammalian pathogenesis.