High-yield methods for accurate two-alternative visual psychophysics in head-fixed mice
High-yield methods for accurate two-alternative visual psychophysics in head-fixed mice
复制标题
头部固定小鼠精确两种视觉心理物理学的高产方法
DOI：
10.1101/051912
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Burgess C
中科院分区：
文献类型：
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作者：
Burgess C
神经科学的研究越来越依赖于老鼠，这是一种哺乳动物，它提供了无与伦比的遗传可塑性和大脑图谱。在这里，我们介绍了高产量的方法探测鼠标的视觉决策。小鼠头部固定，促进可重复的视觉刺激，眼睛跟踪和大脑访问。他们转动方向盘，做出两种选择，强迫或非强迫。由于刺激与车轮位置的直观耦合，学习速度很快。鼠标的决定提供高质量的心理测量曲线检测和歧视，并符合一个简单的概率观察模型的预测。这项任务很容易与皮质活动的双光子成像配对。光遗传失活表明，这项任务需要老鼠使用他们的视觉皮层。通过流体奖励或多巴胺神经元的光遗传学刺激来激励小鼠执行任务。这种刺激激发了更多的尝试和更快的学习。这些方法提供了一个平台，以准确地探测小鼠视觉及其神经基础。
Research in neuroscience increasingly relies on the mouse, a mammalian species that affords unparalleled genetic tractability and brain atlases. Here, we introduce high-yield methods for probing mouse visual decisions. Mice are head-fixed, facilitating repeatable visual stimulation, eye tracking, and brain access. They turn a steering wheel to make two alternative choices, forced or unforced. Learning is rapid thanks to intuitive coupling of stimuli to wheel position. The mouse decisions deliver high-quality psychometric curves for detection and discrimination and conform to the predictions of a simple probabilistic observer model. The task is readily paired with two-photon imaging of cortical activity. Optogenetic inactivation reveals that the task requires mice to use their visual cortex. Mice are motivated to perform the task by fluid reward or optogenetic stimulation of dopamine neurons. This stimulation elicits a larger number of trials and faster learning. These methods provide a platform to accurately probe mouse vision and its neural basis.