Sequence analysis and expression of the human parainfluenza type 1 virus nucleoprotein gene.
Sequence analysis and expression of the human parainfluenza type 1 virus nucleoprotein gene.
复制标题
人副流感1型病毒核蛋白基因的序列分析和表达。
DOI：
10.1016/0042-6822(91)90514-c
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发表时间：
1991
期刊：
影响因子：
3.7
通讯作者：
Ray,R
中科院分区：
文献类型：
--
作者：
Matsuoka,Y;Ray,R
从人副流感病毒1型(1311)病毒核蛋白(NP)基因的cDNA克隆中测定了该基因的核苷酸序列。经部分核苷酸序列分析和与仙台病毒NP的序列比较，确定4-31为PI1 NP特异性克隆。利用痘苗病毒瞬时表达系统，从该克隆合成了一种与PI1病毒多克隆抗体和抗PI1 NP单抗特异结合的多肽，其凝胶迁移率与PI 1 NP相似。测定了该克隆的完整核苷酸序列，发现该克隆含有一个开放阅读框，可编码524个氨基酸的蛋白质，预测相对分子质量为57,547。PI1NP与其他副粘病毒的氨基酸序列比较表明，在其他副粘病毒中也存在两个保守的氨基酸序列。虽然PI-1和SV具有很高的序列同源性，但与其他副粘病毒一样，在C-末端大约有100个氨基酸是高度不同的。
The nucleotide sequence of the human parainfluenza type 1 (1311) virus nucleoprotein (NP) gene was determined from cDNA clones of the mRNA. A cDNA clone, 4–31, containing a 1.7-kb insert, was identified as a PI1 NP-specific clone by partial nucleotide sequence analysis and sequence comparison with Sendai virus (SV) NP. Using a vaccinia virus transient expression system, a polypeptide with electrophoretic mobility similar to that of PI 1 NP was synthesized from this clone, which reacted specifically with both polyclonal and monoclonal antibodies against PI1 virus and PI1 NP, respectively. The complete nucleotide sequence of this clone was determined and was found to contain a single open reading frame that can encode a protein of 524 amino acids with a predicted molecular weight of 57,547. Comparison of the amino acid sequence of PI1 NP with that of other paramyxoviruses showed that two conserved amino acid sequences found within other paramyxoviruses are also present in PI 1 NP. Although PI 1 and SV showed a high sequence homology, approximately 100 amino acids at the C-terminal region were highly divergent as found also among other paramyxoviruses.