Diversity of Escherichia coli strains involved in vertebral osteomyelitis and arthritis in broilers in Brazil.
Diversity of Escherichia coli strains involved in vertebral osteomyelitis and arthritis in broilers in Brazil.
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DOI：
10.1186/s12917-016-0762-0
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发表时间：
2016-07-14
影响因子：
2.6
通讯作者：
Schouler C
中科院分区：
文献类型：
--
作者：
Braga JFV;Chanteloup NK;Trotereau A;Baucheron S;Guabiraba R;Ecco R;Schouler C
运动障碍和大肠杆菌感染是全世界家禽业的主要关注点。禽致病性大肠杆菌 (APEC) 与导致呼吸道或全身性疾病（称为大肠杆菌病）的肠外感染有关。大肠杆菌病最常见的病变是肝周炎、气囊炎、心包炎、腹膜炎/输卵管炎和关节炎。这些疾病给全世界家禽业造成了重大经济损失。最近从巴西的椎骨骨髓炎病例中分离出大肠杆菌，但没有关于从肉鸡运动系统病变中分离出的大肠杆菌菌株的分子和表型特征的数据。这就提出了一个问题：特定的大肠杆菌菌株是否可能导致肉鸡的骨损伤。本研究的目的是评估从巴西患有椎骨骨髓炎和关节炎的肉鸡中分离出的大肠杆菌菌株的这些特征。将来自骨病变的 15 株大肠杆菌提交给 APEC 诊断，并通过脉冲场凝胶电泳 (PFGE) 和多位点序列分型 (MLST) 确定 ECOR 系统发育群、O 血清群、鞭毛类型、毒力基因含量、遗传模式。此外，通过致死性测试、血清耐药性测试和抗生素耐药性分析进一步表征了细菌分离株。根据 PFGE 评估，无论鸡群来源和病变部位如何，大肠杆菌菌株都具有不同的遗传模式。这些菌株属于先前描述的七种序列类型 (ST)（ST117、ST101、ST131、ST 371 和 ST3107）或本研究中新描述的（ST5766 和 ST5856）。 ECOR D 组 (66.7%) 是最常检测到的。这些菌株属于不同的血清群（O88、O25、O12 和 O45），其中一些在世界范围内具有重要意义。抗生素耐药性概况证实了菌株的多样性，并揭示了高比例的多重耐药菌株（73%），主要针对喹诺酮类和 β-内酰胺类药物，包括第三代头孢菌素。对四环素的耐药性百分比中等（33%），但始终与多重耐药性相关。我们的结果表明，根据分子和表型特征，肉鸡的椎骨骨髓炎和关节炎可能与高度多样化的大肠杆菌有关。与椎骨骨髓炎或关节炎相关的大肠杆菌菌株没有特定的毒力模式。此外，大肠杆菌菌株经常具有多重耐药性，属于 APEC 和人类 ExPEC 菌株共有的 ST。本文的在线版本 (doi:10.1186/s12917-016-0762-0) 包含补充材料，可供授权用户使用。
Locomotor disorders and infections by Escherichia coli represent major concerns to the poultry industry worldwide. Avian pathogenic E. coli (APEC) is associated with extraintestinal infections leading to respiratory or systemic disease known as colibacillosis. The most common lesions seen in cases of colibacillosis are perihepatitis, airsacculitis, pericarditis, peritonitis/salpingitis and arthritis. These diseases are responsible for significant economic losses in the poultry industry worldwide. E. coli has been recently isolated from vertebral osteomyelitis cases in Brazil and there are no data on molecular and phenotypic characteristics of E. coli strains isolated from lesions in the locomotor system of broilers. This raised the question whether specific E. coli strains could be responsible for bone lesions in broilers. The aim of this study was to assess these characteristics of E. coli strains isolated from broilers presenting vertebral osteomyelitis and arthritis in Brazil. Fifteen E. coli strains from bone lesions were submitted to APEC diagnosis and setting of ECOR phylogenic group, O serogroup, flagella type, virulence genes content, genetic patterns by Pulsed Field Gel Electrophoresis (PFGE) and Multilocus Sequence Typing (MLST). In addition, bacterial isolates were further characterized through a lethality test, serum resistance test and antibiotic resistance profile. E. coli strains harbored different genetic pattern as assessed by PFGE, regardless of flock origin and lesion site. The strains belonged to seven sequence types (STs) previously described (ST117, ST101, ST131, ST 371 and ST3107) or newly described in this study (ST5766 and ST5856). ECOR group D (66.7 %) was the most frequently detected. The strains belonged to diverse serogroups (O88, O25, O12, and O45), some of worldwide importance. The antibiotic resistance profile confirmed strains’ diversity and revealed a high proportion of multidrug-resistant strains (73 %), mainly to quinolones and beta-lactams, including third generation cephalosporin. The percentage of resistance to tetracycline was moderate (33 %) but always associated with multidrug resistance. Our results demonstrated that vertebral osteomyelitis and arthritis in broilers can be associated with highly diverse E. coli based on molecular and phenotypic characteristics. There was no specific virulence patterns of the E. coli strains associated with vertebral osteomyelitis or arthritis. Also, E. coli strains were frequently multidrug resistant and belonged to STs commonly shared by APEC and human ExPEC strains. The online version of this article (doi:10.1186/s12917-016-0762-0) contains supplementary material, which is available to authorized users.