Variability in spore germination response by strains of proteolytic Clostridium botulinum types A, B and F
Variability in spore germination response by strains of proteolytic Clostridium botulinum types A, B and F
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DOI：
10.1046/j.1472-765x.2003.01260.x
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发表时间：
2003-01-01
影响因子：
2.4
通讯作者：
Peck, MW
中科院分区：
文献类型：
--
作者：
Alberto, F;Broussolle, V;Peck, MW
目的：本研究的目的是评估10株蛋白水解肉毒梭菌的发芽反应的变异性。方法和结果：一个自动化的浊度法被用来跟踪光密度的下降。产蛋白酶的Cl.肉毒杆菌响应于单独的L-丙氨酸而萌发，通过加入L-乳酸盐或碳酸氢盐离子，萌发的速率和程度增加。其他疏水性氨基酸也引发孢子萌发的蛋白水解Cl。肉毒杆菌而不是AGFK和肌苷，枯草芽孢杆菌或B的萌发剂。结论：与非蛋白水解的C1.肉毒杆菌，所有蛋白水解Cl.肉毒杆菌在没有L-乳酸盐的疏水L-氨基酸中萌发。然而，一个很大的变异性的反应，发芽剂之间的物种是证明。研究意义和影响：选择一个模式菌株，研究萌发的Cl。肉毒杆菌孢子应该考虑到在这项工作中显示的对萌发剂的敏感性的可变性。特别是，测序菌株ATCC 3502可能不是发芽研究的最合适模型。
Aims: The objective of the study was to evaluate the variability of germination response of 10 strains of proteolytic Clostridium botulinum .Methods and Results: An automated turbidometric method was used to follow the fall in optical density. Spores of proteolytic Cl. botulinum germinated in response to L-alanine alone, with rate and extent of germination increased by addition of L-lactate or bicarbonate ions. Other hydrophobic amino acids also triggered germination of spores of proteolytic Cl. botulinum but not AGFK and inosine, germinants for Bacillus subtilis or B. cereus .Conclusions: Unlike spores of nonproteolytic Cl. botulinum, all proteolytic Cl. botulinum germinate in hydrophobic L-amino acids without L-lactate. However, a great variability of response to germinant is evidenced between the species.Significance and Impact of the Study: The selection of a model strain to study germination of Cl. botulinum spores should consider the variability in sensitivity to germinants shown in this work. In particular, the sequenced strain ATCC 3502 may not be the most appropriate model for germination studies.