Increased expression of hepatoma-derived growth factor correlates with poor prognosis in human nasopharyngeal carcinoma
Increased expression of hepatoma-derived growth factor correlates with poor prognosis in human nasopharyngeal carcinoma
复制标题
DOI：
10.1111/j.1365-2559.2010.03739.x
复制
发表时间：
2011-01-01
期刊：
影响因子：
6.4
通讯作者：
Fang, Weiyi
中科院分区：
文献类型：
--
作者：
Wang, Shuang;Fang, Weiyi
目的：探讨肝癌源性生长因子（hepatoma-derived growth factor，HDGF）在鼻咽癌组织中的表达与临床病理因素的关系，包括患者生存期。方法：采用实时定量PCR和Western blot方法，检测HDGF在正常鼻咽组织、鼻咽癌组织和鼻咽癌细胞系中的mRNA和蛋白表达。在由160例原发性NPC组织和71例非癌鼻咽组织组成的回顾性系列中，通过免疫组织化学分析进一步确定HDGF水平。鼻咽癌组织中存在HDGF蛋白和mRNA的高表达。免疫组化结果显示，HDGF在鼻咽癌组织中的阳性表达率分别为53.8%（86/160）和19.4%（32/160）。统计学分析显示，核HDGF的高表达与T分期（P = 0.005）和临床分期（P = 0.038）有关，而与淋巴结转移（P = 0.059）和远处转移（P = 0.563）无关。HDGF表达水平升高的患者总生存率低于HDGF表达水平低的患者（P = 0.006）。多因素分析显示，核HDGF的高表达是一个独立的预后指标的患者survival.Conclusions：核HDGF表达增加是一个潜在的不利预后因素鼻咽癌患者。
Aims:To examine the correlation between hepatoma-derived growth factor (HDGF) expression and clinicopathological data in nasopharyngeal carcinoma (NPC), including patient survival.Methods and results:Using real-time polymerase chain reaction (PCR) and Western blot, mRNA and protein expression of HDGF was detected in normal nasopharyngeal tissues, NPC tissues and cell lines. HDGF levels were determined further by an immunohistochemical analysis in a retrospective series consisting of 160 primary NPC tissues and 71 non-cancerous nasopharynx tissues. Overexpressed mRNA and HDGF protein was present in NPC. By immunohistochemical analysis, we found that 53.8% (86 of 160) and 19.4% (32 of 160) of NPC biopsy specimens showed higher HDGF expression of the nucleus and cytoplasm, respectively. Statistical analysis showed that the higher expression of nuclear HDGF was associated significantly with T stage (P = 0.005) and clinical stage (P = 0.038), but there was no association with lymph node (P = 0.059) or distant metastasis (P = 0.563). Patients with increased HDGF expression levels had poorer overall survival rates than those with low expression of HDGF levels (P = 0.006). Multivariate analysis revealed that high expression of nuclear HDGF was an independent prognostic indicator of patient survival.Conclusions:Increased nuclear expression of HDGF is a potential unfavourable prognostic factor for patients with NPC.