In vivo studies of ultrafast near-infrared laser tissue bonding and wound healing.
In vivo studies of ultrafast near-infrared laser tissue bonding and wound healing.
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超快近红外激光组织粘合和伤口愈合的体内研究。
DOI：
10.1117/1.jbo.20.10.108001
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发表时间：
2015
影响因子：
3.5
通讯作者：
Alfano,RobertR
中科院分区：
文献类型：
--
作者：
Sriramoju,Vidyasagar;Alfano,RobertR
与相应的连续波 (CW) 激光器相比，近红外 (NIR) 范围内的飞秒 (fs) 脉冲激光器在与生物分子相互作用时表现出非常独特的特性。超快近红外激光组织粘合（LTB）用于利用飞秒激光光激发染色体的固有振动来融合体内两个相对的动物组织片段的边缘。由于近红外激光辐射与目标组织中的水和结构蛋白（如胶原蛋白）发生能量-物质相互作用，切割组织在体内在分子水平上实现了融合。水和胶原蛋白吸收飞秒激光产生的非热振动激发，导致焊接线两侧的组织蛋白之间形成交联，从而导致组织粘合。没有使用外在试剂来促进 fs LTB 中的组织结合。这些研究的目的是了解和评估超快近红外飞秒激光辐射在 LTB 和随后的伤口愈合中的作用。 fs LTB 可用于难以缝合的结构，如血管、神经、胆囊、肝脏、肠道和其他内脏。超快近红外 LTB 在最佳条件下的伤口闭合和伤口愈合方面产生了良好的结果和益处。
Femtosecond (fs) pulse lasers in the near-infrared (NIR) range exhibit very distinct properties upon their interaction with biomolecules compared to the corresponding continuous wave (CW) lasers. Ultrafast NIR laser tissue bonding (LTB) was used to fuse edges of two opposing animal tissue segmentsin vivousing fs laser photoexcitation of the native vibrations of chomophores. The fusion of the incised tissues was achievedin vivoat the molecular level as the result of the energy–matter interactions of NIR laser radiation with water and the structural proteins like collagen in the target tissues. Nonthermal vibrational excitation from the fs laser absorption by water and collagen induced the formation of cross-links between tissue proteins on either sides of the weld line resulting in tissue bonding. No extrinsic agents were used to facilitate tissue bonding in the fs LTB. These studies were pursued for the understanding and evaluation of the role of ultrafast NIR fs laser radiation in the LTB and consequent wound healing. The fs LTB can be used for difficult to suture structures such as blood vessels, nerves, gallbladder, liver, intestines, and other viscera. Ultrafast NIR LTB yields promising outcomes and benefits in terms of wound closure and wound healing under optimal conditions.