FUMONISIN-INDUCED AND AAL-TOXIN-INDUCED DISRUPTION OF SPHINGOLIPID METABOLISM WITH ACCUMULATION OF FREE SPHINGOID BASES
FUMONISIN-INDUCED AND AAL-TOXIN-INDUCED DISRUPTION OF SPHINGOLIPID METABOLISM WITH ACCUMULATION OF FREE SPHINGOID BASES
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DOI：
10.1104/pp.106.3.1085
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发表时间：
1994-11-01
期刊：
影响因子：
7.4
通讯作者：
RILEY, RT
中科院分区：
文献类型：
--
作者：
ABBAS, HK;TANAKA, T;RILEY, RT
伏马毒素（FB）和aal毒素是由几种与植物疾病相关的真菌产生的鞘状化合物。在动物细胞中，由念珠菌镰刀菌产生的伏马毒素和由番茄赤霉产生的aal毒素抑制神经酰胺合成，这是与食用念珠菌污染的玉米有关的动物疾病的早期生化事件。在浮萍（Lemna pausicostata Heglem. 6746）、番茄（Lycopersicon esculentum Mill）和烟草愈伤组织（Nicotiana tabacum cv Wisconsin）中，纯FB1或aal毒素引起鞘氨酸和鞘氨酸的显著升高，鞘氨酸和鞘氨酸通常以低浓度存在。三种植物体系的相对增幅差异较大。尽管如此，鞘脂代谢的破坏显然是暴露于FB1或aal毒素的植物的共同特征。抗病番茄品种（Asc/Asc）对毒素诱导的游离鞘氨酸增加的敏感性要低得多。由于游离鞘碱是植物“神经酰胺”的前体，它们的积累表明，主要的生化损伤是抑制新生神经酰胺合成和游离鞘碱的再酰化。因此，在植物中，与交替假单胞杆菌和念珠菌感染相关的疾病症状可能是由于鞘脂代谢的破坏。
Fumonisins (FB) and AAL-toxin are sphingoid-like compounds produced by several species of fungi associated with plant diseases. In animal cells, both fumonisins produced by Fusarium moniliforme and AAL-toxin produced by Alternaria alternate f. sp. lycopersici inhibit ceramide synthesis, an early biochemical event in the animal diseases associated with consumption of F. moniliforme-contaminated corn. In duckweed (Lemna pausicostata Heglem. 6746), tomato plants (Lycopersicon esculentum Mill), and tobacco callus (Nicotiana tabacum cv Wisconsin), pure FB1 or AAL-toxin caused a marked elevation of phytosphingosine and sphinganine, sphingoid bases normally present in low concentrations. The relative increases were quite different in the three plant systems. Nonetheless, disruption of sphingolipid metabolism was clearly a common feature in plants exposed to FB1 or AAL-toxin. Resistant varieties of tomato (Asc/Asc) were much less sensitive to toxin-induced increases in free sphinganine. Because free sphingoid bases are precursors to plant ''ceramides,'' their accumulation suggests that the primary biochemical lesion is inhibition of de novo ceramide synthesis and reacylation of free sphingoid bases. Thus, in plants the disease symptoms associated with A. alternate and F. moniliforme infection may be due to disruption of sphingolipid metabolism.