A Molecular Analysis of Fecal and Mucosal Bacterial Communities in Irritable Bowel Syndrome
A Molecular Analysis of Fecal and Mucosal Bacterial Communities in Irritable Bowel Syndrome
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DOI：
10.1007/s10620-009-0934-x
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发表时间：
2010-02-01
影响因子：
3.1
通讯作者：
Marchesi, Julian R.
中科院分区：
文献类型：
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作者：
Codling, Caroline;O'Mahony, Liam;Marchesi, Julian R.
目的本研究的目的是，首先，使用非培养方法确定肠易激综合征（IBS）患者肠道菌群的多样性（16S rRNA基因的DGGE），其次，检查IBS患者的粘膜活检组织，并将其与自身粪便微生物群进行比较。方法将IBS患者粪便材料中优势微生物群的多样性与健康对照进行比较组结果47例肠易激综合征患者与33例健康对照者肠道菌群相似性差异有统计学意义（P < 0.001），其中健康对照者肠道菌群的变异性明显大于肠易激综合征患者。粘膜和管腔微生物群之间的平均个体内相似性为84%，这表明两个位点存在不同的群落。然而，这种差异与先前在对照受试者中描述的这两个小生境之间的差异相似。肠易激综合征患者肠黏膜和肠腔细菌群落的平均个体间相似性差异无统计学意义（P > 0.05）。结论肠易激综合征对肠易激综合征患者肠黏膜和肠腔细菌群落的影响程度相同，肠易激综合征患者和健康对照者的肠道菌群存在显著差异。这种差异的原因尚不清楚，有各种可能的解释，但需要做更多的工作来确定这种观察结果的根本原因。
Purpose The objectives of this study were, firstly, to determine the diversity of the host's gut microbiota in irritable bowel syndrome (IBS) using a culture-independent method (DGGE of the 16S rRNA gene) and, secondly, to examine mucosal biopsies of IBS patients and compare them to their own fecal microbiota.Methods The diversity of the dominant microbiota in the fecal material of IBS patients was compared to a healthy control group. In addition, we compared the mucosal and fecal microbiota of IBS patients.Results Statistical analysis of the mean similarity data for these groups indicated a significant difference (P < 0.001) between IBS (n = 47) and healthy controls (n = 33) with significantly more variation in the gut microbiota of healthy volunteers than that of IBS patients. The average intra-individual similarity between the mucosa and luminal microbiota was 84%, which indicates that different communities were present at the two sites. This difference, however, is similar to that previously described between these two niches in control subjects. The average inter-individual similarity of the bacterial communities on the mucosa and in the lumen of IBS was not significantly different (P > 0.05).Conclusions IBS impacts equally on both bacterial communities in the IBS host and a significant difference in the gut microbiota exists between fecal samples from IBS patients and healthy controls. The reason for this difference is unclear and various possible explanations are available, but much more work is required to determine the underlying reason for this observation.