High glucose increases growth and collagen synthesis in cultured human tubulointerstitial cells
High glucose increases growth and collagen synthesis in cultured human tubulointerstitial cells
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DOI：
10.1046/j.1464-5491.1999.00174.x
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发表时间：
1999-11-01
影响因子：
3.5
通讯作者：
Pollock, CA
中科院分区：
文献类型：
--
作者：
Jones, SC;Saunders, HJ;Pollock, CA
目的改变近端肾小管细胞生长和间质纤维化是糖尿病肾病的特征，并与疾病进展相关。这些观察结果知之甚少，尽管有人认为它们是继发于肾小球疾病的。本研究的主要目的是评估高细胞外葡萄糖浓度对人小管间质的直接影响。方法将人近端小管细胞（ptc）和皮质成纤维细胞（CFs）在含葡萄糖6.1 mol/l和25mmol/l的培养基中培养6 d。测定细胞增殖、胸苷摄取（DNA合成的标志）、蛋白质含量和胶原合成。结果在ptc中，暴露于高葡萄糖与细胞数量增加410 +/- 108%相关（P < 0.001）；每细胞胸苷摄取增加了101 +/- 24% (P < 0.01)，每细胞蛋白质含量降低了39 +/- 6% （P < 0.05）。胶原合成增加了37 +/- 11% (P
Aims Altered proximal tubular cell growth and interstitial fibrosis are features of diabetic nephropathy and correlate with disease progression. These observations are poorly understood, although it has been suggested that they are secondary to glomerular disease. The primary aim of this study was to assess the direct effects of high extracellular glucose concentrations on the human tubulointerstitium.Methods Primary cultures of human proximal tubule cells (PTCs) and cortical fibroblasts (CFs) were grown for 6 days in media containing either 6.1 mol/l or 25mmol/l glucose. Cell proliferation, thymidine uptake (a marker-of DNA synthesis), protein content and collagen synthesis were measured.Results In PTCs, exposure to high glucose was associated with a 410 +/- 108% increase in cell numbers (P < 0.001); 101 +/- 24% increase in thymidine uptake per cell (P < 0.01) and a 39 +/ -6% decease in protein content per cell (P < 0.05). Collagen synthesis was increased by 37 +/- 11% (P