Productive infection of primary human endothelial cells by pig endogenous retrovirus (PERV)
Productive infection of primary human endothelial cells by pig endogenous retrovirus (PERV)
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DOI：
10.1034/j.1399-3089.2000.00052.x
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发表时间：
2000-05-01
影响因子：
3.9
通讯作者：
Steinhoff, G
中科院分区：
文献类型：
--
作者：
Martin, U;Winkler, ME;Steinhoff, G
异种移植中病毒传播的潜在风险引起了人们的广泛关注。不同的猪细胞类型已经被证明可以释放逆转录病毒颗粒，这种颗粒在体外对人类细胞系具有传染性。然而，关于PERV（猪内源性逆转录病毒）是否能够感染人类原代细胞的数据很少。在这项研究中，我们分析了内皮细胞、血管成纤维细胞、系膜细胞、单核细胞、造血干细胞和骨髓基质细胞对PERV传播的影响。我们现在提供的证据表明，原代人内皮细胞、血管成纤维细胞和系膜细胞易受PERV传播。PERV感染在内皮细胞和系膜细胞中产生。我们的数据证实并扩展了以前关于PERV感染人类细胞的报道。不同原代人细胞的PERV感染为异种移植期间的病毒风险提供了进一步的重要证据。在这种情况下，应特别注意人内皮细胞的生产性感染：在异种移植的情况下，这种细胞类型将与猪细胞和PERV颗粒密切接触。
The potential risk of viral transmission in the setting of xenotransplantation has gained major attention. Different porcine cell types have been shown to release retroviral particles, which are infectious for human cell lines in vitro. However, there are only a few data on whether PERV (pig endogenous retrovirus) is able to infect primary human cells. In this study we have analyzed endothelial cells, vascular fibroblasts, mesangial cells, mononuclear cells, hematopoetic stem cells and bone marrow stromal cells for PERV transmission. We now provide evidence for primary human endothelial cells, vascular fibroblasts, and mesangial cells to be susceptible to PERV transmission. PERV infection was productive in endothelial cells and mesangial cells. Our data confirm and extend former reports concerning the PERV infection of human cells. The PERV infection of different primary human cells represents further significant evidence for a viral risk during xenotransplantation. In this context, special attention should be directed towards productive infection of human endothelial cells: in the setting of xenotransplantation this cell type will have close contact with porcine cells and PERV particles.